Kaldenhoff, Ralf ; Russo, V. E. (1993)
Promoter analysis of the bli-7/eas gene.
In: Current genetics, 24 (5)
Article, Bibliographie
Abstract
Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between -1498 bp and -1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between -595 bp and -429 bp, as well as a stretch of DNA from -429 bp to -380 bp which may exert a positive influence after light induction.
| Item Type: | Article |
|---|---|
| Erschienen: | 1993 |
| Creators: | Kaldenhoff, Ralf ; Russo, V. E. |
| Type of entry: | Bibliographie |
| Title: | Promoter analysis of the bli-7/eas gene. |
| Language: | English |
| Date: | 1993 |
| Journal or Publication Title: | Current genetics |
| Volume of the journal: | 24 |
| Issue Number: | 5 |
| Abstract: | Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between -1498 bp and -1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between -595 bp and -429 bp, as well as a stretch of DNA from -429 bp to -380 bp which may exert a positive influence after light induction. |
| Divisions: | 10 Department of Biology > Applied Plant Sciences ?? fb10_botanik ?? 10 Department of Biology |
| Date Deposited: | 31 Aug 2011 13:18 |
| Last Modified: | 05 Mar 2013 09:54 |
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