Kuhse, J. ; Laube, Bodo ; Magalei, D. ; Betz, H. (1993)
Assembly of the inhibitory glycine receptor: identification of amino acid sequence motifs governing subunit stoichiometry.
In: Neuron, 11 (6)
Article, Bibliographie
Abstract
The inhibitory glycine receptor (GlyR) is a pentameric protein composed of two types (alpha and beta) of membrane-spanning subunits. Coexpression in Xenopus oocytes of a low affinity mutant of the alpha 2 subunit with the alpha 1 and beta subunits indicated that GlyRs assembled from alpha 1 and alpha 2 polypeptides contain variable subunit ratios, whereas alpha/beta hetero-oligomers have an invariant (3:2) stoichiometry. Analysis of different alpha/beta chimeric constructs revealed that this difference in assembly behavior is mediated by the N-terminal extracellular regions of the receptor subunits. Substitution of residues diverging between the alpha and beta subunits identified combinations of sequence motifs determining subunit stoichiometry.
| Item Type: | Article |
|---|---|
| Erschienen: | 1993 |
| Creators: | Kuhse, J. ; Laube, Bodo ; Magalei, D. ; Betz, H. |
| Type of entry: | Bibliographie |
| Title: | Assembly of the inhibitory glycine receptor: identification of amino acid sequence motifs governing subunit stoichiometry. |
| Language: | English |
| Date: | 1993 |
| Journal or Publication Title: | Neuron |
| Volume of the journal: | 11 |
| Issue Number: | 6 |
| Abstract: | The inhibitory glycine receptor (GlyR) is a pentameric protein composed of two types (alpha and beta) of membrane-spanning subunits. Coexpression in Xenopus oocytes of a low affinity mutant of the alpha 2 subunit with the alpha 1 and beta subunits indicated that GlyRs assembled from alpha 1 and alpha 2 polypeptides contain variable subunit ratios, whereas alpha/beta hetero-oligomers have an invariant (3:2) stoichiometry. Analysis of different alpha/beta chimeric constructs revealed that this difference in assembly behavior is mediated by the N-terminal extracellular regions of the receptor subunits. Substitution of residues diverging between the alpha and beta subunits identified combinations of sequence motifs determining subunit stoichiometry. |
| Divisions: | 10 Department of Biology 10 Department of Biology > Neurophysiology and Neurosensory Systems ?? fb10_zoologie ?? |
| Date Deposited: | 12 Apr 2011 11:27 |
| Last Modified: | 05 Mar 2019 06:48 |
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