TU Darmstadt / ULB / TUbiblio

Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA.

Dueckershoff, K. and Unger, M. and Frank, A. and Gillam, E. M. J. and Guengerich, F. P. and Warzecha, Heribert (2005):
Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA.
In: FEBS letters, 579 (11), pp. 2480-4. ISSN 0014-5793,
[Article]

Abstract

In this study, the human cytochrome P450 (CYP) 2A6 was used in order to modify the alkaloid production of tobacco plants. The cDNA for human CYP2A6 was placed under the control of the constitutive 35S promoter and transferred into Nicotiana tabacum via Agrobacterium-mediated transformation. Transgenic plants showed formation of the recombinant CYP2A6 enzyme but no obvious phenotypic changes. Unlike wild-type tobacco, the transgenic plants accumulated cotinine, a metabolite which is usually formed from nicotine in humans. This result substantiates that metabolic engineering of the plant secondary metabolism via mammalian P450 enzymes is possible in vivo.

Item Type: Article
Erschienen: 2005
Creators: Dueckershoff, K. and Unger, M. and Frank, A. and Gillam, E. M. J. and Guengerich, F. P. and Warzecha, Heribert
Title: Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA.
Language: English
Abstract:

In this study, the human cytochrome P450 (CYP) 2A6 was used in order to modify the alkaloid production of tobacco plants. The cDNA for human CYP2A6 was placed under the control of the constitutive 35S promoter and transferred into Nicotiana tabacum via Agrobacterium-mediated transformation. Transgenic plants showed formation of the recombinant CYP2A6 enzyme but no obvious phenotypic changes. Unlike wild-type tobacco, the transgenic plants accumulated cotinine, a metabolite which is usually formed from nicotine in humans. This result substantiates that metabolic engineering of the plant secondary metabolism via mammalian P450 enzymes is possible in vivo.

Journal or Publication Title: FEBS letters
Journal volume: 579
Number: 11
Divisions: 10 Department of Biology > Plant Biotechnology and Metabolic Engineering
?? fb10_botanik ??
10 Department of Biology
Date Deposited: 17 Mar 2011 11:03
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details