Dueckershoff, K. ; Unger, M. ; Frank, A. ; Gillam, E. M. J. ; Guengerich, F. P. ; Warzecha, Heribert (2005)
Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA.
In: FEBS letters, 579 (11)
Article, Bibliographie
Abstract
In this study, the human cytochrome P450 (CYP) 2A6 was used in order to modify the alkaloid production of tobacco plants. The cDNA for human CYP2A6 was placed under the control of the constitutive 35S promoter and transferred into Nicotiana tabacum via Agrobacterium-mediated transformation. Transgenic plants showed formation of the recombinant CYP2A6 enzyme but no obvious phenotypic changes. Unlike wild-type tobacco, the transgenic plants accumulated cotinine, a metabolite which is usually formed from nicotine in humans. This result substantiates that metabolic engineering of the plant secondary metabolism via mammalian P450 enzymes is possible in vivo.
| Item Type: | Article |
|---|---|
| Erschienen: | 2005 |
| Creators: | Dueckershoff, K. ; Unger, M. ; Frank, A. ; Gillam, E. M. J. ; Guengerich, F. P. ; Warzecha, Heribert |
| Type of entry: | Bibliographie |
| Title: | Modified nicotine metabolism in transgenic tobacco plants expressing the human cytochrome P450 2A6 cDNA. |
| Language: | English |
| Date: | 2005 |
| Journal or Publication Title: | FEBS letters |
| Volume of the journal: | 579 |
| Issue Number: | 11 |
| Abstract: | In this study, the human cytochrome P450 (CYP) 2A6 was used in order to modify the alkaloid production of tobacco plants. The cDNA for human CYP2A6 was placed under the control of the constitutive 35S promoter and transferred into Nicotiana tabacum via Agrobacterium-mediated transformation. Transgenic plants showed formation of the recombinant CYP2A6 enzyme but no obvious phenotypic changes. Unlike wild-type tobacco, the transgenic plants accumulated cotinine, a metabolite which is usually formed from nicotine in humans. This result substantiates that metabolic engineering of the plant secondary metabolism via mammalian P450 enzymes is possible in vivo. |
| Divisions: | 10 Department of Biology > Plant Biotechnology and Metabolic Engineering ?? fb10_botanik ?? 10 Department of Biology |
| Date Deposited: | 17 Mar 2011 11:03 |
| Last Modified: | 05 Mar 2013 09:46 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Send an inquiry |
Options (only for editors)
 |
Show editorial Details |
Drucken
Impressum