Leonhardt, H. ; Page, A. W. ; Weier, H. U. ; Bestor, T. H. (1992)
A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei.
In: Cell, 71 (5)
Article, Bibliographie
Abstract
Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but to display a diffuse nucleoplasmic distribution in non-S phase cells. Analysis of DNA MTase-beta-galactosidase fusion proteins has shown that association with replication foci is mediated by a novel targeting sequence located near the N-terminus of DNA MTase. This sequence has the properties expected of a targeting sequence in that it is not required for enzymatic activity, prevents proper targeting when deleted, and, when fused to beta-galactosidase, causes the fusion protein to associate with replication foci in a cell cycle-dependent manner.
| Item Type: | Article |
|---|---|
| Erschienen: | 1992 |
| Creators: | Leonhardt, H. ; Page, A. W. ; Weier, H. U. ; Bestor, T. H. |
| Type of entry: | Bibliographie |
| Title: | A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei. |
| Language: | English |
| Date: | 1992 |
| Journal or Publication Title: | Cell |
| Volume of the journal: | 71 |
| Issue Number: | 5 |
| URL / URN: | http://www.cardoso-lab.org/publications/Leonhardt_1992.pdf |
| Abstract: | Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but to display a diffuse nucleoplasmic distribution in non-S phase cells. Analysis of DNA MTase-beta-galactosidase fusion proteins has shown that association with replication foci is mediated by a novel targeting sequence located near the N-terminus of DNA MTase. This sequence has the properties expected of a targeting sequence in that it is not required for enzymatic activity, prevents proper targeting when deleted, and, when fused to beta-galactosidase, causes the fusion protein to associate with replication foci in a cell cycle-dependent manner. |
| Divisions: | 10 Department of Biology ?? fb10_zoologie ?? 10 Department of Biology > Cell Biology and Epigenetics |
| Date Deposited: | 05 Mar 2010 14:54 |
| Last Modified: | 17 Dec 2018 15:19 |
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