Sporbert, Anje ; Gahl, Anja ; Ankerhold, Richard ; Leonhardt, Heinrich ; Cardoso, M. Cristina (2002)
DNA polymerase clamp shows little turnover at established replication sites but sequential de novo assembly at adjacent origin clusters.
In: Molecular cell, 10 (6)
Article, Bibliographie
Abstract
The spatial and temporal organization of DNA replication was investigated in living cells with a green fluorescent protein fusion to the DNA polymerase clamp PCNA. In situ extractions and photobleaching experiments revealed that PCNA, unlike RPA34, shows little if any turnover at replication sites, suggesting that it remains associated with the replication machinery through multiple rounds of Okazaki fragment synthesis. Photobleaching analyses further showed that the transition from earlier to later replicons occurs by disassembly into a nucleoplasmic pool of rapidly diffusing subcomponents and reassembly at newly activated sites. The fact that these replication sites were de novo assembled in close proximity to earlier ones suggests that activation of neighboring origins may occur by a domino effect possibly involving local changes in chromatin structure and accessibility.
| Item Type: | Article |
|---|---|
| Erschienen: | 2002 |
| Creators: | Sporbert, Anje ; Gahl, Anja ; Ankerhold, Richard ; Leonhardt, Heinrich ; Cardoso, M. Cristina |
| Type of entry: | Bibliographie |
| Title: | DNA polymerase clamp shows little turnover at established replication sites but sequential de novo assembly at adjacent origin clusters. |
| Language: | German |
| Date: | 2002 |
| Journal or Publication Title: | Molecular cell |
| Volume of the journal: | 10 |
| Issue Number: | 6 |
| URL / URN: | http://www.cardoso-lab.org/publications/Sporbert_2002+Suppl.... |
| Abstract: | The spatial and temporal organization of DNA replication was investigated in living cells with a green fluorescent protein fusion to the DNA polymerase clamp PCNA. In situ extractions and photobleaching experiments revealed that PCNA, unlike RPA34, shows little if any turnover at replication sites, suggesting that it remains associated with the replication machinery through multiple rounds of Okazaki fragment synthesis. Photobleaching analyses further showed that the transition from earlier to later replicons occurs by disassembly into a nucleoplasmic pool of rapidly diffusing subcomponents and reassembly at newly activated sites. The fact that these replication sites were de novo assembled in close proximity to earlier ones suggests that activation of neighboring origins may occur by a domino effect possibly involving local changes in chromatin structure and accessibility. |
| Divisions: | 10 Department of Biology > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Department of Biology |
| Date Deposited: | 06 Mar 2010 07:21 |
| Last Modified: | 05 Mar 2013 09:32 |
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