Grohmann, Maik ; Spada, Fabio ; Schermelleh, Lothar ; Alenina, Natalia ; Bader, Michael ; Cardoso, M. Cristina ; Leonhardt, Heinrich (2005)
Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming.
In: BMC developmental biology, 5
Article, Bibliographie
Abstract
BACKGROUND: Mouse preimplantation development is characterized by both active and passive genomic demethylation. A short isoform of the prevalent maintenance DNA methyltransferase (Dnmt1S) is found in the cytoplasm of preimplantation embryos and transiently enters the nucleus only at the 8-cell stage. RESULTS: Using GFP fusions we show that both the long and short isoforms of Dnmt1 localize to the nucleus of somatic cells and the cytoplasm of preimplantation embryos and that these subcellular localization properties are independent of phosphorylation. Importantly, photobleaching techniques and salt extraction revealed that Dnmt1S has a very restricted mobility in the cytoplasm, while it is highly mobile in the nucleus of preimplantation embryos. CONCLUSION: The restricted mobility of Dnmt1S limits its access to DNA and likely contributes to passive demethylation and epigenetic reprogramming during preimplantation development.
| Item Type: | Article |
|---|---|
| Erschienen: | 2005 |
| Creators: | Grohmann, Maik ; Spada, Fabio ; Schermelleh, Lothar ; Alenina, Natalia ; Bader, Michael ; Cardoso, M. Cristina ; Leonhardt, Heinrich |
| Type of entry: | Bibliographie |
| Title: | Restricted mobility of Dnmt1 in preimplantation embryos: implications for epigenetic reprogramming. |
| Language: | German |
| Date: | 2005 |
| Journal or Publication Title: | BMC developmental biology |
| Volume of the journal: | 5 |
| URL / URN: | http://www.cardoso-lab.org/publications/Grohmann_et_al_2005.... |
| Abstract: | BACKGROUND: Mouse preimplantation development is characterized by both active and passive genomic demethylation. A short isoform of the prevalent maintenance DNA methyltransferase (Dnmt1S) is found in the cytoplasm of preimplantation embryos and transiently enters the nucleus only at the 8-cell stage. RESULTS: Using GFP fusions we show that both the long and short isoforms of Dnmt1 localize to the nucleus of somatic cells and the cytoplasm of preimplantation embryos and that these subcellular localization properties are independent of phosphorylation. Importantly, photobleaching techniques and salt extraction revealed that Dnmt1S has a very restricted mobility in the cytoplasm, while it is highly mobile in the nucleus of preimplantation embryos. CONCLUSION: The restricted mobility of Dnmt1S limits its access to DNA and likely contributes to passive demethylation and epigenetic reprogramming during preimplantation development. |
| Divisions: | 10 Department of Biology > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Department of Biology |
| Date Deposited: | 06 Mar 2010 07:50 |
| Last Modified: | 05 Mar 2013 09:32 |
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