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Targeting and tracing antigens in live cells with fluorescent nanobodies.

Rothbauer, Ulrich ; Zolghadr, Kourosh ; Tillib, Sergei ; Nowak, Danny ; Schermelleh, Lothar ; Gahl, Anja ; Backmann, Natalija ; Conrath, Katja ; Muyldermans, Serge ; Cardoso, M. Cristina ; Leonhardt, Heinrich (2006):
Targeting and tracing antigens in live cells with fluorescent nanobodies.
In: Nature methods, 3 (11), pp. 887-9. ISSN 1548-7091,
[Article]

Abstract

We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.

Item Type: Article
Erschienen: 2006
Creators: Rothbauer, Ulrich ; Zolghadr, Kourosh ; Tillib, Sergei ; Nowak, Danny ; Schermelleh, Lothar ; Gahl, Anja ; Backmann, Natalija ; Conrath, Katja ; Muyldermans, Serge ; Cardoso, M. Cristina ; Leonhardt, Heinrich
Title: Targeting and tracing antigens in live cells with fluorescent nanobodies.
Language: German
Abstract:

We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.

Journal or Publication Title: Nature methods
Journal volume: 3
Number: 11
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 08:16
Official URL: http://www.cardoso-lab.org/publications/Rothbauer%20et%20al....
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