Rothbauer, Ulrich ; Zolghadr, Kourosh ; Tillib, Sergei ; Nowak, Danny ; Schermelleh, Lothar ; Gahl, Anja ; Backmann, Natalija ; Conrath, Katja ; Muyldermans, Serge ; Cardoso, M. Cristina ; Leonhardt, Heinrich (2006)
Targeting and tracing antigens in live cells with fluorescent nanobodies.
In: Nature methods, 3 (11)
Article, Bibliographie
Abstract
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
| Item Type: | Article |
|---|---|
| Erschienen: | 2006 |
| Creators: | Rothbauer, Ulrich ; Zolghadr, Kourosh ; Tillib, Sergei ; Nowak, Danny ; Schermelleh, Lothar ; Gahl, Anja ; Backmann, Natalija ; Conrath, Katja ; Muyldermans, Serge ; Cardoso, M. Cristina ; Leonhardt, Heinrich |
| Type of entry: | Bibliographie |
| Title: | Targeting and tracing antigens in live cells with fluorescent nanobodies. |
| Language: | German |
| Date: | 2006 |
| Journal or Publication Title: | Nature methods |
| Volume of the journal: | 3 |
| Issue Number: | 11 |
| URL / URN: | http://www.cardoso-lab.org/publications/Rothbauer%20et%20al.... |
| Corresponding Links: | |
| Abstract: | We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion. |
| Divisions: | 10 Department of Biology > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Department of Biology |
| Date Deposited: | 06 Mar 2010 08:16 |
| Last Modified: | 05 Mar 2013 09:32 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Send an inquiry |
Options (only for editors)
 |
Show editorial Details |
Drucken
Impressum