Schermelleh, Lothar ; Carlton, Peter M. ; Haase, Sebastian ; Shao, Lin ; Winoto, Lukman ; Kner, Peter ; Burke, Brian ; Cardoso, M. Cristina ; Agard, David A. ; Gustafsson, Mats G. L. ; Leonhardt, Heinrich ; Sedat, John W. (2008)
Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.
In: Science, 320 (5881)
Article, Bibliographie
Abstract
Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.
| Item Type: | Article |
|---|---|
| Erschienen: | 2008 |
| Creators: | Schermelleh, Lothar ; Carlton, Peter M. ; Haase, Sebastian ; Shao, Lin ; Winoto, Lukman ; Kner, Peter ; Burke, Brian ; Cardoso, M. Cristina ; Agard, David A. ; Gustafsson, Mats G. L. ; Leonhardt, Heinrich ; Sedat, John W. |
| Type of entry: | Bibliographie |
| Title: | Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy. |
| Language: | English |
| Date: | 2008 |
| Journal or Publication Title: | Science |
| Volume of the journal: | 320 |
| Issue Number: | 5881 |
| URL / URN: | http://www.cardoso-lab.org/publications/Schermelleh_2008.pdf |
| Corresponding Links: | |
| Abstract: | Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light. |
| Divisions: | 10 Department of Biology > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Department of Biology |
| Date Deposited: | 06 Mar 2010 15:57 |
| Last Modified: | 05 Mar 2013 09:32 |
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