Hassan, Mohamed Atef ; Kern, Michal ; Chu, Anh ; Kalra, Gatik ; Shabratova, Ekaterina ; Tsarapkin, Aleksei ; MacKinnon, Neil ; Lips, Klaus ; Teutloff, Christian ; Bittl, Robert ; Korvink, Jan Gerrit ; Anders, Jens (2022)
Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors.
In: Frequenz, 76 (11-12)
doi: 10.1515/freq-2022-0096
Article, Bibliographie
This is the latest version of this item.
Abstract
Electron paramagnetic resonance (EPR) is the gold standard for studying paramagnetic species. As an example, in structural biology, it allows to extract information about distance distributions on the nanometer scale via site-directed spin labeling. Conventional pulsed EPR of biological samples is currently limited to relatively large sample concentrations and cryogenic temperatures, mainly due to low sensitivity and the significant dead time associated with conventional resonator-based EPR setups, essentially precluding in-cell EPR under physiological conditions. This paper presents our latest progress toward single-cell pulsed EPR using VCO-based EPR-on-a-chip (EPRoC) sensors. Together with an analytical model for VCO-based pulsed EPR, we present an experimental scheme to perform dead time- free pulsed EPR measurements using EPRoC detectors. The proposed scheme is validated using extensive numerical simulations and proof-of-concept experiments on the spin dynamics of an organic radical at room temperature using a custom-designed EPRoC detector operating in the Ka-band around 30.4 GHz. Additionally, we discuss methods to improve the excitation field homogeneity and sample handling through chip post-processing and custom-designed microfluidics. Finally, we present our progress towards compact, portable pulsed EPR spectrometers incorporating EPRoC detectors, microfluidics, and custom-designed permanent magnets. Such portable EPR spectrometers can pave the way toward new EPR applications, including point-of care diagnostics.
| Item Type: | Article |
|---|---|
| Erschienen: | 2022 |
| Creators: | Hassan, Mohamed Atef ; Kern, Michal ; Chu, Anh ; Kalra, Gatik ; Shabratova, Ekaterina ; Tsarapkin, Aleksei ; MacKinnon, Neil ; Lips, Klaus ; Teutloff, Christian ; Bittl, Robert ; Korvink, Jan Gerrit ; Anders, Jens |
| Type of entry: | Bibliographie |
| Title: | Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors |
| Language: | English |
| Date: | 2022 |
| Place of Publication: | Darmstadt |
| Publisher: | De Gruyter |
| Journal or Publication Title: | Frequenz |
| Volume of the journal: | 76 |
| Issue Number: | 11-12 |
| DOI: | 10.1515/freq-2022-0096 |
| Corresponding Links: | |
| Abstract: | Electron paramagnetic resonance (EPR) is the gold standard for studying paramagnetic species. As an example, in structural biology, it allows to extract information about distance distributions on the nanometer scale via site-directed spin labeling. Conventional pulsed EPR of biological samples is currently limited to relatively large sample concentrations and cryogenic temperatures, mainly due to low sensitivity and the significant dead time associated with conventional resonator-based EPR setups, essentially precluding in-cell EPR under physiological conditions. This paper presents our latest progress toward single-cell pulsed EPR using VCO-based EPR-on-a-chip (EPRoC) sensors. Together with an analytical model for VCO-based pulsed EPR, we present an experimental scheme to perform dead time- free pulsed EPR measurements using EPRoC detectors. The proposed scheme is validated using extensive numerical simulations and proof-of-concept experiments on the spin dynamics of an organic radical at room temperature using a custom-designed EPRoC detector operating in the Ka-band around 30.4 GHz. Additionally, we discuss methods to improve the excitation field homogeneity and sample handling through chip post-processing and custom-designed microfluidics. Finally, we present our progress towards compact, portable pulsed EPR spectrometers incorporating EPRoC detectors, microfluidics, and custom-designed permanent magnets. Such portable EPR spectrometers can pave the way toward new EPR applications, including point-of care diagnostics. |
| Uncontrolled Keywords: | dead time free EPR, EPR, EPR-on-a-chip, EPRoC, Rabi oscillations, VCO-based pulsed EPR |
| Classification DDC: | 500 Science and mathematics > 530 Physics 500 Science and mathematics > 570 Life sciences, biology 600 Technology, medicine, applied sciences > 620 Engineering and machine engineering |
| Divisions: | 18 Department of Electrical Engineering and Information Technology 18 Department of Electrical Engineering and Information Technology > Institute for Microwave Engineering and Photonics (IMP) |
| Date Deposited: | 02 Aug 2024 12:50 |
| Last Modified: | 02 Aug 2024 12:50 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
Available Versions of this Item
-
Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors. (deposited 01 Mar 2023 10:02)
- Towards single-cell pulsed EPR using VCO-based EPR-on-a-chip detectors. (deposited 02 Aug 2024 12:50) [Currently Displayed]
 |
Send an inquiry |
Options (only for editors)
 |
Show editorial Details |
Drucken
Impressum