Ghosh, Eashika ; Egunov, Aleksandr I. ; Karnaushenko, Daniil ; Medina-Sánchez, Mariana ; Schmidt, Oliver G. (2022)
Self-assembled sensor-in-a-tube as a versatile tool for label-free EIS viability investigation of cervical cancer cells.
In: Frequenz, 76 (11-12)
doi: 10.1515/freq-2022-0090
Article, Bibliographie
This is the latest version of this item.
Abstract
The advancement of micro and nanotechnology has led to the manufacturing of miniaturized sensors with improved functionalities for highly sensitive point of care devices. This work is particularly focused on analysing cancer cells and the effect of a model drug on their survival rate. To that end, we developed a highly sensitive rolled-up micro-electrochemical impedance spectroscopy sensor, encapsulated into a microfluidic channel. The sensor was built by strain engineering of shapeable materials and with diameters close to the cell size to improve their sensitivity. To demonstrate the platform performance, we first carried out measurements with different electrode geometries using cell medium at different concentrations. We also performed measurements using cancer cell suspensions, obtaining distinct signals from single cells, cell clusters and cellular debris. Finally, cancer cells were treated with an anticancer drug (Camptothecin), at different concentrations, over the same period, and further analysed using the developed platform.
| Item Type: | Article |
|---|---|
| Erschienen: | 2022 |
| Creators: | Ghosh, Eashika ; Egunov, Aleksandr I. ; Karnaushenko, Daniil ; Medina-Sánchez, Mariana ; Schmidt, Oliver G. |
| Type of entry: | Bibliographie |
| Title: | Self-assembled sensor-in-a-tube as a versatile tool for label-free EIS viability investigation of cervical cancer cells |
| Language: | English |
| Date: | 2022 |
| Place of Publication: | Darmstadt |
| Publisher: | De Gruyter |
| Journal or Publication Title: | Frequenz |
| Volume of the journal: | 76 |
| Issue Number: | 11-12 |
| DOI: | 10.1515/freq-2022-0090 |
| Corresponding Links: | |
| Abstract: | The advancement of micro and nanotechnology has led to the manufacturing of miniaturized sensors with improved functionalities for highly sensitive point of care devices. This work is particularly focused on analysing cancer cells and the effect of a model drug on their survival rate. To that end, we developed a highly sensitive rolled-up micro-electrochemical impedance spectroscopy sensor, encapsulated into a microfluidic channel. The sensor was built by strain engineering of shapeable materials and with diameters close to the cell size to improve their sensitivity. To demonstrate the platform performance, we first carried out measurements with different electrode geometries using cell medium at different concentrations. We also performed measurements using cancer cell suspensions, obtaining distinct signals from single cells, cell clusters and cellular debris. Finally, cancer cells were treated with an anticancer drug (Camptothecin), at different concentrations, over the same period, and further analysed using the developed platform. |
| Uncontrolled Keywords: | electrical impedance spectroscopy, lab-in-atube, label-free impedimetric sensor, shapeable materials technologies, single-cell analysis |
| Classification DDC: | 500 Science and mathematics > 530 Physics 500 Science and mathematics > 570 Life sciences, biology 600 Technology, medicine, applied sciences > 620 Engineering and machine engineering |
| Divisions: | 18 Department of Electrical Engineering and Information Technology 18 Department of Electrical Engineering and Information Technology > Institute for Microwave Engineering and Photonics (IMP) |
| Date Deposited: | 02 Aug 2024 12:50 |
| Last Modified: | 02 Aug 2024 12:50 |
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| Suche nach Titel in: | TUfind oder in Google |
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Self-assembled sensor-in-a-tube as a versatile tool for label-free EIS viability investigation of cervical cancer cells. (deposited 01 Mar 2023 09:58)
- Self-assembled sensor-in-a-tube as a versatile tool for label-free EIS viability investigation of cervical cancer cells. (deposited 02 Aug 2024 12:50) [Currently Displayed]
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