Eckert, Denise ; Rapp, Felicitas ; Tsedeke, Ayele T. ; Molendowska, Jessica ; Lehn, Robert ; Langhans, Markus ; Fournier, Claudia ; Rödel, Franz ; Hehlgans, Stephanie (2022)
ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells.
In: Cells, 11 (1)
doi: 10.3390/cells11010072
Article, Bibliographie
This is the latest version of this item.
Abstract
Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.
| Item Type: | Article |
|---|---|
| Erschienen: | 2022 |
| Creators: | Eckert, Denise ; Rapp, Felicitas ; Tsedeke, Ayele T. ; Molendowska, Jessica ; Lehn, Robert ; Langhans, Markus ; Fournier, Claudia ; Rödel, Franz ; Hehlgans, Stephanie |
| Type of entry: | Bibliographie |
| Title: | ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells |
| Language: | English |
| Date: | 2022 |
| Publisher: | MDPI |
| Journal or Publication Title: | Cells |
| Volume of the journal: | 11 |
| Issue Number: | 1 |
| Collation: | 20 Seiten |
| DOI: | 10.3390/cells11010072 |
| Corresponding Links: | |
| Abstract: | Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source. |
| Uncontrolled Keywords: | adhesion, endothelial cells, inflammation, leukocytes, low-dose irradiation, shear stress |
| Classification DDC: | 500 Science and mathematics > 530 Physics 500 Science and mathematics > 540 Chemistry 500 Science and mathematics > 570 Life sciences, biology 600 Technology, medicine, applied sciences > 610 Medicine and health |
| Divisions: | 10 Department of Biology 10 Department of Biology > Membrane Dynamics 07 Department of Chemistry 07 Department of Chemistry > Ernst-Berl-Institut 07 Department of Chemistry > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie 07 Department of Chemistry > Ernst-Berl-Institut > Fachgebiet Technische Chemie |
| Date Deposited: | 02 Aug 2024 12:40 |
| Last Modified: | 02 Aug 2024 12:40 |
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| Suche nach Titel in: | TUfind oder in Google |
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ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells. (deposited 22 Apr 2022 12:17)
- ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells. (deposited 02 Aug 2024 12:40) [Currently Displayed]
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