Saul, Meike J. ; Hegewald, Anett B. ; Emmerich, Anne C. ; Ossipova, Elena ; Vogel, Marc ; Baumann, Isabell ; Kultima, Kim ; Lengqivst, Johan ; Steinhilber, Dieter ; Jakobsson, Per Johan (2019)
Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes.
In: Frontiers in Pharmacology, 10
doi: 10.3389/fphar.2019.00640
Article, Bibliographie
This is the latest version of this item.
Abstract
MicroRNAs (miRs) are small noncoding RNAs which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR functions. The recent discovery that miR-328 has a noncanonical function and can activate gene expression by antagonizing the activity of heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored and exciting field of gene expression regulation. The global importance of such noncanonical miR function is not yet known. In order to achieve a better understanding of the new miR activity, we performed a compartment specific tandem mass tag (TMT)-based proteomic analysis in differentiated MonoMac6 (MM6) cells, to monitor gene expression variations in response to miR-328 knockdown. We identified a broad spectrum of novel potential miR-328/hnRNP E2 and miR-328 targets involved in regulation of compartment specific cellular processes, such as inflammation or RNA splicing. This study provides first insights of the global significance of noncanonical miR function.
| Item Type: | Article |
|---|---|
| Erschienen: | 2019 |
| Creators: | Saul, Meike J. ; Hegewald, Anett B. ; Emmerich, Anne C. ; Ossipova, Elena ; Vogel, Marc ; Baumann, Isabell ; Kultima, Kim ; Lengqivst, Johan ; Steinhilber, Dieter ; Jakobsson, Per Johan |
| Type of entry: | Bibliographie |
| Title: | Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes |
| Language: | English |
| Date: | 2019 |
| Publisher: | Frontiers |
| Journal or Publication Title: | Frontiers in Pharmacology |
| Volume of the journal: | 10 |
| DOI: | 10.3389/fphar.2019.00640 |
| URL / URN: | https://doi.org/10.3389/fphar.2019.00640 |
| Corresponding Links: | |
| Abstract: | MicroRNAs (miRs) are small noncoding RNAs which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR functions. The recent discovery that miR-328 has a noncanonical function and can activate gene expression by antagonizing the activity of heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored and exciting field of gene expression regulation. The global importance of such noncanonical miR function is not yet known. In order to achieve a better understanding of the new miR activity, we performed a compartment specific tandem mass tag (TMT)-based proteomic analysis in differentiated MonoMac6 (MM6) cells, to monitor gene expression variations in response to miR-328 knockdown. We identified a broad spectrum of novel potential miR-328/hnRNP E2 and miR-328 targets involved in regulation of compartment specific cellular processes, such as inflammation or RNA splicing. This study provides first insights of the global significance of noncanonical miR function. |
| Classification DDC: | 500 Science and mathematics > 570 Life sciences, biology |
| Divisions: | 10 Department of Biology 10 Department of Biology > Synthetic Genetic Circuits (2020 renamed "Synthetic RNA biology) |
| Date Deposited: | 02 Aug 2024 12:33 |
| Last Modified: | 02 Aug 2024 12:33 |
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| Suche nach Titel in: | TUfind oder in Google |
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Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes. (deposited 14 Jul 2019 19:55)
- Mass Spectrometry-Based Proteomics Approach Characterizes the Dual Functionality of miR-328 in Monocytes. (deposited 02 Aug 2024 12:33) [Currently Displayed]
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