Voos, Patrick ; Fuck, Sebastian ; Weipert, Fabian ; Babel, Laura ; Tandl, Dominique ; Meckel, Tobias ; Hehlgans, Stephanie ; Fournier, Claudia ; Moroni, Anna ; Rödel, Franz ; Thiel, Gerhard (2018)
Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells.
In: Frontiers in Immunology, 9
doi: 10.3389/fimmu.2018.00922
Article, Bibliographie
This is the latest version of this item.
Abstract
Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on immune surveillance of tumor cells and non-malignant cells and can either foster therapy response or side effects/toxicities of radiation therapy. For a better understanding of the mechanisms by which IR modulates T-cell activation and alters functional properties of these immune cells, we exposed human immortalized Jurkat cells and peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 and 5 Gy. This resulted in cellular responses, which are typically observed also in naïve T-lymphocytes in response of T-cell receptor immune stimulation or mitogens. These responses include oscillations of cytosolic Ca2+, an upregulation of CD25 surface expression, interleukin-2 and interferon-γ synthesis, elevated expression of Ca2+ sensitive K+ channels and an increase in cell diameter. The latter was sensitive to inhibition by the immunosuppressant cyclosporine A, Ca2+ buffer BAPTA-AM, and the CDK1-inhibitor RO3306, indicating the involvement of Ca2+-dependent immune activation and radiation-induced cell cycle arrest. Furthermore, on a functional level, Jurkat and PBL cell adhesion to endothelial cells was increased upon radiation exposure and was highly dependent on an upregulation of integrin beta-1 expression and clustering. In conclusion, we here report that IR impacts on immune activation and functional properties of T-lymphocytes that may have implications in both toxic effects and treatment response to combined radiation and immune therapy in cancer patients.
| Item Type: | Article |
|---|---|
| Erschienen: | 2018 |
| Creators: | Voos, Patrick ; Fuck, Sebastian ; Weipert, Fabian ; Babel, Laura ; Tandl, Dominique ; Meckel, Tobias ; Hehlgans, Stephanie ; Fournier, Claudia ; Moroni, Anna ; Rödel, Franz ; Thiel, Gerhard |
| Type of entry: | Bibliographie |
| Title: | Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells |
| Language: | English |
| Date: | 2018 |
| Journal or Publication Title: | Frontiers in Immunology |
| Volume of the journal: | 9 |
| DOI: | 10.3389/fimmu.2018.00922 |
| URL / URN: | https://doi.org/10.3389/fimmu.2018.00922 |
| Corresponding Links: | |
| Abstract: | Impairment or stimulation of the immune system by ionizing radiation (IR) impacts on immune surveillance of tumor cells and non-malignant cells and can either foster therapy response or side effects/toxicities of radiation therapy. For a better understanding of the mechanisms by which IR modulates T-cell activation and alters functional properties of these immune cells, we exposed human immortalized Jurkat cells and peripheral blood lymphocytes (PBL) to X-ray doses between 0.1 and 5 Gy. This resulted in cellular responses, which are typically observed also in naïve T-lymphocytes in response of T-cell receptor immune stimulation or mitogens. These responses include oscillations of cytosolic Ca2+, an upregulation of CD25 surface expression, interleukin-2 and interferon-γ synthesis, elevated expression of Ca2+ sensitive K+ channels and an increase in cell diameter. The latter was sensitive to inhibition by the immunosuppressant cyclosporine A, Ca2+ buffer BAPTA-AM, and the CDK1-inhibitor RO3306, indicating the involvement of Ca2+-dependent immune activation and radiation-induced cell cycle arrest. Furthermore, on a functional level, Jurkat and PBL cell adhesion to endothelial cells was increased upon radiation exposure and was highly dependent on an upregulation of integrin beta-1 expression and clustering. In conclusion, we here report that IR impacts on immune activation and functional properties of T-lymphocytes that may have implications in both toxic effects and treatment response to combined radiation and immune therapy in cancer patients. |
| Classification DDC: | 500 Science and mathematics > 570 Life sciences, biology |
| Divisions: | 10 Department of Biology 10 Department of Biology > Plant Membrane Biophyscis (20.12.23 renamed in Biology of Algae and Protozoa) 10 Department of Biology > Membrane Dynamics |
| Date Deposited: | 02 Aug 2024 12:33 |
| Last Modified: | 02 Aug 2024 12:33 |
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| Suche nach Titel in: | TUfind oder in Google |
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Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells. (deposited 03 Jun 2018 19:55)
- Ionizing Radiation Induces Morphological Changes and Immunological Modulation of Jurkat Cells. (deposited 02 Aug 2024 12:33) [Currently Displayed]
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