Koch, Leon F. ; Best, Tatjana ; Wüstenhagen, Elena ; Adrian, Klaus ; Rammo, Oliver ; Saul, Meike J. (2024)
Novel insights into the isolation of extracellular vesicles by anion exchange chromatography.
In: Frontiers in Bioengineering and Biotechnology, 11
doi: 10.3389/fbioe.2023.1298892
Article, Bibliographie
This is the latest version of this item.
Abstract
Extracellular vesicles (EVs) are membrane structures enclosed by a lipid bilayer that are released into the extracellular space by all types of cells. EVs are involved in many physiological processes by transporting biologically active substances. Interest in EVs for diagnostic biomarker research and therapeutic drug delivery applications has increased in recent years. The realization of the full therapeutic potential of EVs is currently hampered by the lack of a suitable technology for the isolation and purification of EVs for downstream pharmaceutical applications. Anion Exchange Chromatography (AEX) is an established method in which specific charges on the AEX matrix can exploit charges on the surface of EVs and their interactions to provide a productive and scalable separation and purification method. The established AEX method using Eshmuno® Q, a strong tentacle anion exchange resin, was used to demonstrate the principal feasibility of AEX-based isolation and gain insight into isolated EV properties. Using several EV analysis techniques to provide a more detailed insight into EV populations during AEX isolation, we demonstrated that although the composition of CD9/63/81 remained constant for tetraspanin positive EVs, the size distribution and purity changed during elution. Higher salt concentrations eluted larger tetraspanin negative vesicles.
| Item Type: | Article |
|---|---|
| Erschienen: | 2024 |
| Creators: | Koch, Leon F. ; Best, Tatjana ; Wüstenhagen, Elena ; Adrian, Klaus ; Rammo, Oliver ; Saul, Meike J. |
| Type of entry: | Bibliographie |
| Title: | Novel insights into the isolation of extracellular vesicles by anion exchange chromatography |
| Language: | English |
| Date: | 19 January 2024 |
| Place of Publication: | Lausanne |
| Publisher: | Frontiers Media S.A. |
| Journal or Publication Title: | Frontiers in Bioengineering and Biotechnology |
| Volume of the journal: | 11 |
| Collation: | 16 Seiten |
| DOI: | 10.3389/fbioe.2023.1298892 |
| Corresponding Links: | |
| Abstract: | Extracellular vesicles (EVs) are membrane structures enclosed by a lipid bilayer that are released into the extracellular space by all types of cells. EVs are involved in many physiological processes by transporting biologically active substances. Interest in EVs for diagnostic biomarker research and therapeutic drug delivery applications has increased in recent years. The realization of the full therapeutic potential of EVs is currently hampered by the lack of a suitable technology for the isolation and purification of EVs for downstream pharmaceutical applications. Anion Exchange Chromatography (AEX) is an established method in which specific charges on the AEX matrix can exploit charges on the surface of EVs and their interactions to provide a productive and scalable separation and purification method. The established AEX method using Eshmuno® Q, a strong tentacle anion exchange resin, was used to demonstrate the principal feasibility of AEX-based isolation and gain insight into isolated EV properties. Using several EV analysis techniques to provide a more detailed insight into EV populations during AEX isolation, we demonstrated that although the composition of CD9/63/81 remained constant for tetraspanin positive EVs, the size distribution and purity changed during elution. Higher salt concentrations eluted larger tetraspanin negative vesicles. |
| Uncontrolled Keywords: | extracellular vesicles, ion-exchange chromatography, isolation, Eshmuno® Q, downstream processing, scalability, charge-based |
| Identification Number: | Artikel-ID: 1298892 |
| Additional Information: | This article is part of the Research Topic: Manufacturing of Next-Generation Biologics Sec. Bioprocess Engineering |
| Classification DDC: | 500 Science and mathematics > 570 Life sciences, biology 600 Technology, medicine, applied sciences > 610 Medicine and health |
| Divisions: | 10 Department of Biology 10 Department of Biology > Extracellular vesicles / miRNA Research |
| Date Deposited: | 05 Jun 2024 09:41 |
| Last Modified: | 05 Jun 2024 12:19 |
| PPN: | 518841987 |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
Available Versions of this Item
-
Novel insights into the isolation of extracellular vesicles by anion exchange chromatography. (deposited 04 Jun 2024 12:48)
- Novel insights into the isolation of extracellular vesicles by anion exchange chromatography. (deposited 05 Jun 2024 09:41) [Currently Displayed]
 |
Send an inquiry |
Options (only for editors)
 |
Show editorial Details |
Drucken
Impressum