Rauer, Sebastian Bernhard ; Bell, Daniel Josef ; Jain, Puja ; Rahimi, Khosrow ; Felder, Daniel ; Linkhorst, John ; Wessling, Matthias (2022)
Porous PEDOT:PSS Particles and Their Application as Tunable Cell Culture Substrate.
In: Advanced Materials Technologies, 7 (1)
doi: 10.1002/admt.202100836
Article, Bibliographie
Abstract
Due to its biocompatibility, electrical conductivity, and tissue-like elasticity, poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) constitutes a highly promising material regarding the fabrication of smart cell culture substrates. However, until now, high-throughput synthesis of pure PEDOT:PSS geometries was restricted to flat sheets and fibers. In this publication, the first microfluidic process for the synthesis of spherical, highly porous, pure PEDOT:PSS particles of adjustable material properties is presented. The particles are synthesized by the generation of PEDOT:PSS emulsion droplets within a 1-octanol continuous phase and their subsequent coagulation and partial crystallization in an isopropanol (IPA)/sulfuric acid (SA) bath. The process allows to tailor central particle characteristics such as crystallinity, particle diameter, pore size as well as electrochemical and mechanical properties by simply adjusting the IPA:SA ratio during droplet coagulation. To demonstrate the applicability of PEDOT:PSS particles as potential cell culture substrate, cultivations of L929 mouse fibroblast cells and MRC-5 human fibroblast cells are conducted. Both cell lines present exponential growth on PEDOT:PSS particles and reach confluency with cell viabilities above 95 vol.% on culture day 9. Single cell analysis could moreover reveal that mechanotransduction and cell infiltration can be controlled by the adjustment of particle crystallinity.
| Item Type: | Article |
|---|---|
| Erschienen: | 2022 |
| Creators: | Rauer, Sebastian Bernhard ; Bell, Daniel Josef ; Jain, Puja ; Rahimi, Khosrow ; Felder, Daniel ; Linkhorst, John ; Wessling, Matthias |
| Type of entry: | Bibliographie |
| Title: | Porous PEDOT:PSS Particles and Their Application as Tunable Cell Culture Substrate |
| Language: | English |
| Date: | 2022 |
| Publisher: | Wiley |
| Journal or Publication Title: | Advanced Materials Technologies |
| Volume of the journal: | 7 |
| Issue Number: | 1 |
| DOI: | 10.1002/admt.202100836 |
| Abstract: | Due to its biocompatibility, electrical conductivity, and tissue-like elasticity, poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) constitutes a highly promising material regarding the fabrication of smart cell culture substrates. However, until now, high-throughput synthesis of pure PEDOT:PSS geometries was restricted to flat sheets and fibers. In this publication, the first microfluidic process for the synthesis of spherical, highly porous, pure PEDOT:PSS particles of adjustable material properties is presented. The particles are synthesized by the generation of PEDOT:PSS emulsion droplets within a 1-octanol continuous phase and their subsequent coagulation and partial crystallization in an isopropanol (IPA)/sulfuric acid (SA) bath. The process allows to tailor central particle characteristics such as crystallinity, particle diameter, pore size as well as electrochemical and mechanical properties by simply adjusting the IPA:SA ratio during droplet coagulation. To demonstrate the applicability of PEDOT:PSS particles as potential cell culture substrate, cultivations of L929 mouse fibroblast cells and MRC-5 human fibroblast cells are conducted. Both cell lines present exponential growth on PEDOT:PSS particles and reach confluency with cell viabilities above 95 vol.% on culture day 9. Single cell analysis could moreover reveal that mechanotransduction and cell infiltration can be controlled by the adjustment of particle crystallinity. |
| Uncontrolled Keywords: | 4-ethylenedioxythiophene):polystyrene sulfonate, cell culture, microcarrier, microfluidic, particle, poly(3) |
| Divisions: | 16 Department of Mechanical Engineering 16 Department of Mechanical Engineering > Chair for Process Engineering of Electrochemical Systems |
| Date Deposited: | 13 Sep 2023 11:13 |
| Last Modified: | 13 Sep 2023 11:13 |
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