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Biocompatible Micron-Scale Silk Fibers Fabricated by Microfluidic Wet Spinning

Lüken, Arne ; Geiger, Matthias ; Steinbeck, Lea ; Joel, Anna-Christin ; Lampert, Angelika ; Linkhorst, John ; Wessling, Matthias (2021)
Biocompatible Micron-Scale Silk Fibers Fabricated by Microfluidic Wet Spinning.
In: Advanced Healthcare Materials, 10 (20)
doi: 10.1002/adhm.202100898
Article, Bibliographie

Abstract

For successful material deployment in tissue engineering, the material itself, its mechanical properties, and the microscopic geometry of the product are of particular interest. While silk is a widely applied protein-based tissue engineering material with strong mechanical properties, the size and shape of artificially spun silk fibers are limited by existing processes. This study adjusts a microfluidic spinneret to manufacture micron-sized wet-spun fibers with three different materials enabling diverse geometries for tissue engineering applications. The spinneret is direct laser written (DLW) inside a microfluidic polydimethylsiloxane (PDMS) chip using two-photon lithography, applying a novel surface treatment that enables a tight print-channel sealing. Alginate, polyacrylonitrile, and silk fibers with diameters down to 1 µ m are spun, while the spinneret geometry controls the shape of the silk fiber, and the spinning process tailors the mechanical property. Cell-cultivation experiments affirm bio-compatibility and showcase an interplay between the cell-sized fibers and cells. The presented spinning process pushes the boundaries of fiber fabrication toward smaller diameters and more complex shapes with increased surface-to-volume ratio and will substantially contribute to future tailored tissue engineering materials for healthcare applications.

Item Type: Article
Erschienen: 2021
Creators: Lüken, Arne ; Geiger, Matthias ; Steinbeck, Lea ; Joel, Anna-Christin ; Lampert, Angelika ; Linkhorst, John ; Wessling, Matthias
Type of entry: Bibliographie
Title: Biocompatible Micron-Scale Silk Fibers Fabricated by Microfluidic Wet Spinning
Language: English
Date: 2021
Publisher: Wiley
Journal or Publication Title: Advanced Healthcare Materials
Volume of the journal: 10
Issue Number: 20
DOI: 10.1002/adhm.202100898
Abstract:

For successful material deployment in tissue engineering, the material itself, its mechanical properties, and the microscopic geometry of the product are of particular interest. While silk is a widely applied protein-based tissue engineering material with strong mechanical properties, the size and shape of artificially spun silk fibers are limited by existing processes. This study adjusts a microfluidic spinneret to manufacture micron-sized wet-spun fibers with three different materials enabling diverse geometries for tissue engineering applications. The spinneret is direct laser written (DLW) inside a microfluidic polydimethylsiloxane (PDMS) chip using two-photon lithography, applying a novel surface treatment that enables a tight print-channel sealing. Alginate, polyacrylonitrile, and silk fibers with diameters down to 1 µ m are spun, while the spinneret geometry controls the shape of the silk fiber, and the spinning process tailors the mechanical property. Cell-cultivation experiments affirm bio-compatibility and showcase an interplay between the cell-sized fibers and cells. The presented spinning process pushes the boundaries of fiber fabrication toward smaller diameters and more complex shapes with increased surface-to-volume ratio and will substantially contribute to future tailored tissue engineering materials for healthcare applications.

Uncontrolled Keywords: 2-photon lithography, additive manufacturing, material properties, tissue engineering
Divisions: 16 Department of Mechanical Engineering
16 Department of Mechanical Engineering > Chair for Process Engineering of Electrochemical Systems
Date Deposited: 13 Sep 2023 11:13
Last Modified: 13 Sep 2023 11:13
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