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1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5'-UTR of SARS-CoV-2

Schnieders, Robbin ; Peter, Stephen A. ; Banijamali, Elnaz ; Riad, Magdalena ; Altincekic, Nadide ; Bains, Jasleen Kaur ; Ceylan, Betül ; Fürtig, Boris ; Grün, J. Tassilo ; Hengesbach, Martin ; Hohmann, Katharina F. ; Hymon, Daniel ; Knezic, Bozana ; Oxenfarth, Andreas ; Petzold, Katja ; Qureshi, Nusrat S. ; Richter, Christian ; Schlagnitweit, Judith ; Schlundt, Andreas ; Schwalbe, Harald ; Stirnal, Elke ; Sudakov, Alexey ; Vögele, Jennifer ; Wacker, Anna ; Weigand, Julia E. ; Wirmer-Bartoschek, Julia ; Wöhnert, Jens (2021)
1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5'-UTR of SARS-CoV-2.
In: Biomolecular NMR assignments, 2021
doi: 10.1007/s12104-021-10007-w
Article, Bibliographie

Abstract

The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5'-untranslated region (5'-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5'-UUUCGU-3' hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive H, C and N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.

Item Type: Article
Erschienen: 2021
Creators: Schnieders, Robbin ; Peter, Stephen A. ; Banijamali, Elnaz ; Riad, Magdalena ; Altincekic, Nadide ; Bains, Jasleen Kaur ; Ceylan, Betül ; Fürtig, Boris ; Grün, J. Tassilo ; Hengesbach, Martin ; Hohmann, Katharina F. ; Hymon, Daniel ; Knezic, Bozana ; Oxenfarth, Andreas ; Petzold, Katja ; Qureshi, Nusrat S. ; Richter, Christian ; Schlagnitweit, Judith ; Schlundt, Andreas ; Schwalbe, Harald ; Stirnal, Elke ; Sudakov, Alexey ; Vögele, Jennifer ; Wacker, Anna ; Weigand, Julia E. ; Wirmer-Bartoschek, Julia ; Wöhnert, Jens
Type of entry: Bibliographie
Title: 1H, 13C and 15N chemical shift assignment of the stem-loop 5a from the 5'-UTR of SARS-CoV-2
Language: English
Date: 23 January 2021
Publisher: Springer
Journal or Publication Title: Biomolecular NMR assignments
Volume of the journal: 2021
DOI: 10.1007/s12104-021-10007-w
URL / URN: https://link.springer.com/article/10.1007/s12104-021-10007-w...
Abstract:

The SARS-CoV-2 (SCoV-2) virus is the causative agent of the ongoing COVID-19 pandemic. It contains a positive sense single-stranded RNA genome and belongs to the genus of Betacoronaviruses. The 5'- and 3'-genomic ends of the 30 kb SCoV-2 genome are potential antiviral drug targets. Major parts of these sequences are highly conserved among Betacoronaviruses and contain cis-acting RNA elements that affect RNA translation and replication. The 31 nucleotide (nt) long highly conserved stem-loop 5a (SL5a) is located within the 5'-untranslated region (5'-UTR) important for viral replication. SL5a features a U-rich asymmetric bulge and is capped with a 5'-UUUCGU-3' hexaloop, which is also found in stem-loop 5b (SL5b). We herein report the extensive H, C and N resonance assignment of SL5a as basis for in-depth structural studies by solution NMR spectroscopy.

Identification Number: pmid:33484403
Divisions: 10 Department of Biology
10 Department of Biology > RNA Biochemistry
Date Deposited: 05 Mar 2021 08:45
Last Modified: 05 Mar 2021 08:45
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