TU Darmstadt / ULB / TUbiblio

A spatial and functional interaction of a heterotetramer Survivin-DNA-PKcs complex in DNA damage response.

Güllülü, Ömer ; Hehlgans, Stephanie ; Mayer, Benjamin E. ; Gößner, Ines ; Petraki, Chrysi ; Hoffmann, Melanie ; Dombrowsky, Maximilian J. ; Kunzmann, Patrick ; Hamacher, Kay ; Strebhardt, Klaus ; Fokas, Emmanouil ; Rödel, Claus ; Münch, Christian ; Rödel, Franz (2021):
A spatial and functional interaction of a heterotetramer Survivin-DNA-PKcs complex in DNA damage response.
In: Cancer research, 81 (9), pp. 2304-2317. ISSN 1538-7445,
DOI: 10.1158/0008-5472.CAN-20-2931,
[Article]

Abstract

Substantial evidence has shown that overexpression of the inhibitor of apoptosis protein (IAP) Survivin in human tumors correlates significantly with treatment resistance and poor patient prognosis. Survivin serves as a radiation resistance factor that impacts the DNA damage response by interacting with DNA-dependent protein kinase (DNA-PKcs). However, the complexity, molecular determinants and functional consequences of this interrelationship remain largely unknown. By applying co-immunoprecipitation and flow cytometry-based Förster resonance energy transfer assays, we demonstrated a direct involvement of the Survivin baculovirus IAP repeat (BIR) domain in the regulation of radiation survival and DNA repair. This Survivin-mediated activity required an interaction of residues S20 and W67 with the phosphoinositide 3-kinase (PI3K) domain of DNA-PKcs. In silico molecular docking and dynamics simulation analyses, in vitro kinase assays, and large-scale mass spectrometry suggested a heterotetrameric Survivin-DNA-PKcs complex that results in a conformational change within the DNA-PKcs PI3K domain. Overexpression or depletion of Survivin resulted in enhanced PI3K enzymatic activity and detection of differentially abundant phosphopeptides and proteins implicated in the DNA damage response. The Survivin-DNA-PKcs interaction altered the S/T-hydrophobic motif substrate specificity of DNA-PKcs with a predominant usage of S/T-P phosphorylation sites and an increase of DNA-PKcs substrates including Foxo3. These data demonstrate that Survivin differentially regulates DNA-PKcs-dependent radiation survival and DNA double-strand break repair via formation of a Survivin-DNA-PKcs heterotetrameric complex.

Item Type: Article
Erschienen: 2021
Creators: Güllülü, Ömer ; Hehlgans, Stephanie ; Mayer, Benjamin E. ; Gößner, Ines ; Petraki, Chrysi ; Hoffmann, Melanie ; Dombrowsky, Maximilian J. ; Kunzmann, Patrick ; Hamacher, Kay ; Strebhardt, Klaus ; Fokas, Emmanouil ; Rödel, Claus ; Münch, Christian ; Rödel, Franz
Title: A spatial and functional interaction of a heterotetramer Survivin-DNA-PKcs complex in DNA damage response.
Language: English
Abstract:

Substantial evidence has shown that overexpression of the inhibitor of apoptosis protein (IAP) Survivin in human tumors correlates significantly with treatment resistance and poor patient prognosis. Survivin serves as a radiation resistance factor that impacts the DNA damage response by interacting with DNA-dependent protein kinase (DNA-PKcs). However, the complexity, molecular determinants and functional consequences of this interrelationship remain largely unknown. By applying co-immunoprecipitation and flow cytometry-based Förster resonance energy transfer assays, we demonstrated a direct involvement of the Survivin baculovirus IAP repeat (BIR) domain in the regulation of radiation survival and DNA repair. This Survivin-mediated activity required an interaction of residues S20 and W67 with the phosphoinositide 3-kinase (PI3K) domain of DNA-PKcs. In silico molecular docking and dynamics simulation analyses, in vitro kinase assays, and large-scale mass spectrometry suggested a heterotetrameric Survivin-DNA-PKcs complex that results in a conformational change within the DNA-PKcs PI3K domain. Overexpression or depletion of Survivin resulted in enhanced PI3K enzymatic activity and detection of differentially abundant phosphopeptides and proteins implicated in the DNA damage response. The Survivin-DNA-PKcs interaction altered the S/T-hydrophobic motif substrate specificity of DNA-PKcs with a predominant usage of S/T-P phosphorylation sites and an increase of DNA-PKcs substrates including Foxo3. These data demonstrate that Survivin differentially regulates DNA-PKcs-dependent radiation survival and DNA double-strand break repair via formation of a Survivin-DNA-PKcs heterotetrameric complex.

Journal or Publication Title: Cancer research
Journal Volume: 81
Issue Number: 9
Divisions: 10 Department of Biology
10 Department of Biology > Computational Biology and Simulation
Date Deposited: 11 Jan 2021 14:05
DOI: 10.1158/0008-5472.CAN-20-2931
Identification Number: pmid:33408118
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details