Nasir, Saima ; Ali, Mubarak ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang (2020)
Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates.
In: ChemPlusChem, 85 (3)
doi: 10.1002/cplu.202000045
Article, Bibliographie
This is the latest version of this item.
Abstract
We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (>= 1 nM) of phosphoprotein (albumin and alpha-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho-alpha-casein even at higher concentration (>1 mu M) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins.
| Item Type: | Article |
|---|---|
| Erschienen: | 2020 |
| Creators: | Nasir, Saima ; Ali, Mubarak ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang |
| Type of entry: | Bibliographie |
| Title: | Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates |
| Language: | English |
| Date: | March 2020 |
| Publisher: | Wiley-VCH |
| Journal or Publication Title: | ChemPlusChem |
| Volume of the journal: | 85 |
| Issue Number: | 3 |
| DOI: | 10.1002/cplu.202000045 |
| URL / URN: | https://doi.org/10.1002/cplu.202000045 |
| Corresponding Links: | |
| Abstract: | We report a nanofluidic device for the label-free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, namely 4-[bis(2-pyridylmethyl)aminomethyl]aniline (DPA-NH2) compound was synthesized. Single asymmetric nanofluidic channels were fabricated in polyethylene terephthalate (PET) membranes. The chelator (DPA-NH2) molecules are subsequently immobilized on the nanochannel surface, followed by the zinc ion complexation to afford DPA-Zn2+ chelates, which act as ligand moieties for the specific binding of phosphoproteins. The success of the chemical reaction and biomolecular recognition process that occur in a confined geometry can be monitored from the changes in electrical readout of the nanochannel. The nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (>= 1 nM) of phosphoprotein (albumin and alpha-casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. However, dephosphoproteins such as lysozyme and dephospho-alpha-casein even at higher concentration (>1 mu M) could not induce any significant change in the transmembrane ion flux. This observation indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins, and has potential for use in differentiating between phosphoproteins and dephosphoproteins. |
| Uncontrolled Keywords: | iomolecules, ion current rectification, ligand-receptor interactions, sensors, synthetic nanochannels |
| Divisions: | 11 Department of Materials and Earth Sciences 11 Department of Materials and Earth Sciences > Material Science 11 Department of Materials and Earth Sciences > Material Science > Material Analytics |
| Date Deposited: | 16 Nov 2020 06:21 |
| Last Modified: | 03 Jul 2024 02:48 |
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| Suche nach Titel in: | TUfind oder in Google |
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Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates. (deposited 05 Apr 2020 19:57)
- Phosphoprotein Detection with a Single Nanofluidic Diode Decorated with Zinc Chelates. (deposited 16 Nov 2020 06:21) [Currently Displayed]
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