Ali, Mubarak ; Nasir, Saima ; Ahmed, Ishtiaq ; Niemeyer, Christof M. ; Ensinger, Wolfgang (2020)
Biomolecular Detection with a Single Nanofluidic Diode Decorated with Metal Chelates.
In: ChemPlusChem
doi: 10.1002/cplu.202000045
Article, Bibliographie

Abstract

Here we demonstrate a nanofluidic device for the label‐free detection of phosphoprotein (PPn) analytes. To achieve this goal, a metal ion chelator, i.e., the 4‐[bis(2‐pyridylmethyl)aminomethyl]aniline (DPA–NH2) compound is synthesized. Single asymmetric nanofluidic channels are fabricated in polyethylene terephthalate (PET) membranes. Then the chelator (DPA–NH2) molecules are immobilized on the nanochannel surface followed by the zinc ion complexation to afford DPA–Zn2+ chelates which act as ligand moieties for the specific binding of phosphoproteins. The success of chemical reaction and biomolecular recognition process occurring in confined geometry is monitored from the changes in electrical readout of the nanochannel. The designed nanofluidic sensor has the ability to sensitively and specifically detect lower concentrations (≥1 nM) of phosphoprotein (albumin and α‐casein) in the surrounding environment as evidenced from the significant decrease in ion current flowing through the nanochannels. While, dephosphoproteins such as lysozyme and dephospho‐α‐casein even at higher concentration (˃1 µM) could not induce any significant change in the transmembrane ion flux. This indicated the sensitivity and specificity of the proposed nanofluidic sensor towards PPn proteins. In this context, we believe that metal affinity‐based nanofluidic sensor would readily be used to differentiate in between phosphoproteins and dephosphoproteins

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