TU Darmstadt / ULB / TUbiblio

Targeted Manipulation/Repositioning of Subcellular Structures and Molecules.

Heinz, Kathrin S. ; Cardoso, M. Cristina (2019):
Targeted Manipulation/Repositioning of Subcellular Structures and Molecules.
In: Methods in molecular biology (Clifton, N.J.), 2038, pp. 199-208. ISSN 1940-6029,
DOI: 10.1007/978-1-4939-9674-2_13,
[Article]

Abstract

Technical advances in live-cell imaging have made cell biology into a highly dynamic field, allowing the visualization and quantification of complex processes in individual cells and in real time. To follow changes and to specifically manipulate factors potentially involved in processes like DNA replication, transcription or repair, we set up a universal targeting approach, allowing directed manipulation of subcellular structures and molecules therein. This strategy is based on the very strong and specific interaction of GFP and GFP-binding nanobody. We describe in detail how to set up the targeting approach with appropriate controls, as well as how to improve and validate its efficiency and finally provide exemplary applications.

Item Type: Article
Erschienen: 2019
Creators: Heinz, Kathrin S. ; Cardoso, M. Cristina
Title: Targeted Manipulation/Repositioning of Subcellular Structures and Molecules.
Language: English
Abstract:

Technical advances in live-cell imaging have made cell biology into a highly dynamic field, allowing the visualization and quantification of complex processes in individual cells and in real time. To follow changes and to specifically manipulate factors potentially involved in processes like DNA replication, transcription or repair, we set up a universal targeting approach, allowing directed manipulation of subcellular structures and molecules therein. This strategy is based on the very strong and specific interaction of GFP and GFP-binding nanobody. We describe in detail how to set up the targeting approach with appropriate controls, as well as how to improve and validate its efficiency and finally provide exemplary applications.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Journal volume: 2038
Divisions: 10 Department of Biology
10 Department of Biology > Cell Biology and Epigenetics
Date Deposited: 26 Aug 2019 09:30
DOI: 10.1007/978-1-4939-9674-2_13
Identification Number: pmid:31407286
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)
Show editorial Details Show editorial Details