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RNA-based Capture-SELEX for the selection of small molecule-binding aptamers.

Boussebayle, Adrien ; Groher, Florian ; Suess, Beatrix (2019):
RNA-based Capture-SELEX for the selection of small molecule-binding aptamers.
In: Methods (San Diego, Calif.), 161, pp. 10-15. ISSN 1095-9130,
DOI: 10.1016/j.ymeth.2019.04.004,
[Article]

Abstract

Despite their wide applicability, the selection of small molecule-binding RNA aptamers with both high affinity binding and specificity is still challenging. Aptamers that excel at both binding and structure switching are particularly rare and difficult to find. Here, we present the protocol of a Capture-SELEX that specifically allows the in vitro selection of small-molecule binding aptamers, which are essential building blocks for the design process of synthetic riboswitches and biosensors. Moreover, we provide a comparative overview of our proposed methodology versus alternative in vitro selection protocols with a special focus on the design of the pool. Finally, we have included detailed notes to point out useful tips and pitfalls for future application.

Item Type: Article
Erschienen: 2019
Creators: Boussebayle, Adrien ; Groher, Florian ; Suess, Beatrix
Title: RNA-based Capture-SELEX for the selection of small molecule-binding aptamers.
Language: English
Abstract:

Despite their wide applicability, the selection of small molecule-binding RNA aptamers with both high affinity binding and specificity is still challenging. Aptamers that excel at both binding and structure switching are particularly rare and difficult to find. Here, we present the protocol of a Capture-SELEX that specifically allows the in vitro selection of small-molecule binding aptamers, which are essential building blocks for the design process of synthetic riboswitches and biosensors. Moreover, we provide a comparative overview of our proposed methodology versus alternative in vitro selection protocols with a special focus on the design of the pool. Finally, we have included detailed notes to point out useful tips and pitfalls for future application.

Journal or Publication Title: Methods (San Diego, Calif.)
Journal volume: 161
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 15 Apr 2019 09:47
DOI: 10.1016/j.ymeth.2019.04.004
Identification Number: pmid:30953759
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