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Assessing the influence of biofilm surface roughness on mass transfer by combining optical coherence tomography and two-dimensional modeling

Li, Chunyan and Wagner, Michael and Lackner, Susanne and Horn, Harald (2016):
Assessing the influence of biofilm surface roughness on mass transfer by combining optical coherence tomography and two-dimensional modeling.
In: Biotechnology and Bioengineering, pp. 989-1000, 113, (5), ISSN 0006-3592,
DOI: 10.1002/bit.25868,
[Online-Edition: https://www.ncbi.nlm.nih.gov/pubmed/26498328],
[Article]

Abstract

Imaging and modeling are two major approaches in biofilm research to understand the physical and biochemical processes involved in biofilm development. However, they are often used separately. In this study we combined these two approaches to investigate substrate mass transfer and mass flux. Cross-sectional biofilm images were acquired by means of optical coherence tomography (OCT) for biofilms grown on carriers. A 2D biofilm model was developed incorporating OCT images as well as a simplified biofilm geometry serving as structural templates. The model incorporated fluid flow, substrate transfer and biochemical conversion of substrates and simulated the hydrodynamics surrounding the biofilm structure as well as the substrate distribution. The method allowed detailed analysis of the hydrodynamics and mass transfer characteristics at the micro-scale. Biofilm activity with respect to substrate fluxes was compared among different combinations of flow, substrate availability and biomass density. The combined approach revealed that higher substrate fluxes at heterogeneous biofilm surface under two conditions: pure diffusion and when high flow velocity along the biofilms surface renders the whole liquid-biofilm interface to be highly active. In-between the two conditions the substrate fluxes across the surface of smooth biofilm geometry were higher than that of the heterogeneous biofilms. Biotechnol. Bioeng. 2016;113: 989-1000. (c) 2015 Wiley Periodicals, Inc.

Item Type: Article
Erschienen: 2016
Creators: Li, Chunyan and Wagner, Michael and Lackner, Susanne and Horn, Harald
Title: Assessing the influence of biofilm surface roughness on mass transfer by combining optical coherence tomography and two-dimensional modeling
Language: English
Abstract:

Imaging and modeling are two major approaches in biofilm research to understand the physical and biochemical processes involved in biofilm development. However, they are often used separately. In this study we combined these two approaches to investigate substrate mass transfer and mass flux. Cross-sectional biofilm images were acquired by means of optical coherence tomography (OCT) for biofilms grown on carriers. A 2D biofilm model was developed incorporating OCT images as well as a simplified biofilm geometry serving as structural templates. The model incorporated fluid flow, substrate transfer and biochemical conversion of substrates and simulated the hydrodynamics surrounding the biofilm structure as well as the substrate distribution. The method allowed detailed analysis of the hydrodynamics and mass transfer characteristics at the micro-scale. Biofilm activity with respect to substrate fluxes was compared among different combinations of flow, substrate availability and biomass density. The combined approach revealed that higher substrate fluxes at heterogeneous biofilm surface under two conditions: pure diffusion and when high flow velocity along the biofilms surface renders the whole liquid-biofilm interface to be highly active. In-between the two conditions the substrate fluxes across the surface of smooth biofilm geometry were higher than that of the heterogeneous biofilms. Biotechnol. Bioeng. 2016;113: 989-1000. (c) 2015 Wiley Periodicals, Inc.

Journal or Publication Title: Biotechnology and Bioengineering
Volume: 113
Number: 5
Uncontrolled Keywords: biofilm imaging optical coherence tomography biofilm modeling mass transfer convection diffusion hydrodynamic conditions finite-element image-analysis detachment microscopy systems transport adhesion flux Biotechnology & Applied Microbiology
Divisions: 13 Department of Civil and Environmental Engineering Sciences > Institute IWAR > Wastewater Engineering
13 Department of Civil and Environmental Engineering Sciences > Institute IWAR
13 Department of Civil and Environmental Engineering Sciences
Date Deposited: 11 Apr 2018 08:32
DOI: 10.1002/bit.25868
Official URL: https://www.ncbi.nlm.nih.gov/pubmed/26498328
Additional Information:

ISI Document Delivery No.: DI4NN Times Cited: 3 Cited Reference Count: 36 Li, Chunyan Wagner, Michael Lackner, Susanne Horn, Harald Horn, Harald/H-1650-2013 Horn, Harald/0000-0002-9385-3883 AnoxKaldnes; Water Science Alliance of The Helmholtz Association The authors acknowledge the support of AnoxKaldnes. Financial support from the Water Science Alliance of The Helmholtz Association is also acknowledged. 3 7 38 Wiley-blackwell Hoboken 1097-0290

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