Rauh, O. ; Hansen, U. P. ; Mach, S. ; Hartel, A. J. W. ; Shepard, K. L. ; Thiel, Gerhard ; Schroeder, Indra (2017)
Extended beta distributions open the access to fast gating in bilayer experiments: assigning the voltage-dependent gating to the selectivity filter.
In: FEBS letters, 591 (23)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Lipid bilayers provide many benefits for ion channel recordings, such as control of membrane composition and transport molecules. However, they suffer from high membrane capacitance limiting the bandwidth and impeding analysis of fast gating. This can be overcome by fitting the deviations of the open channel noise from the baseline noise by extended beta distributions. We demonstrate this analysis step-by-step by applying it to the example of viral K(+) channels (Kcv), from the choice of the gating model through the fitting process, validation of the results, and what kinds of results can be obtained. These voltage sensor-less channels show profoundly voltage-dependent gating with dwell times in the closed state of about 50 μs. Mutations assign it to the selectivity filter. This article is protected by copyright. All rights reserved.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2017 |
| Autor(en): | Rauh, O. ; Hansen, U. P. ; Mach, S. ; Hartel, A. J. W. ; Shepard, K. L. ; Thiel, Gerhard ; Schroeder, Indra |
| Art des Eintrags: | Bibliographie |
| Titel: | Extended beta distributions open the access to fast gating in bilayer experiments: assigning the voltage-dependent gating to the selectivity filter. |
| Sprache: | Englisch |
| Publikationsjahr: | Dezember 2017 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 591 |
| (Heft-)Nummer: | 23 |
| Kurzbeschreibung (Abstract): | Lipid bilayers provide many benefits for ion channel recordings, such as control of membrane composition and transport molecules. However, they suffer from high membrane capacitance limiting the bandwidth and impeding analysis of fast gating. This can be overcome by fitting the deviations of the open channel noise from the baseline noise by extended beta distributions. We demonstrate this analysis step-by-step by applying it to the example of viral K(+) channels (Kcv), from the choice of the gating model through the fitting process, validation of the results, and what kinds of results can be obtained. These voltage sensor-less channels show profoundly voltage-dependent gating with dwell times in the closed state of about 50 μs. Mutations assign it to the selectivity filter. This article is protected by copyright. All rights reserved. |
| ID-Nummer: | pmid:29106736 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 14 Nov 2017 10:33 |
| Letzte Änderung: | 03 Jan 2018 10:29 |
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