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Extended beta distributions open the access to fast gating in bilayer experiments: assigning the voltage-dependent gating to the selectivity filter.

Rauh, O. and Hansen, U. P. and Mach, S. and Hartel, A. J. W. and Shepard, K. L. and Thiel, Gerhard and Schroeder, Indra (2017):
Extended beta distributions open the access to fast gating in bilayer experiments: assigning the voltage-dependent gating to the selectivity filter.
In: FEBS letters, pp. 3850-3860, 591, (23), ISSN 1873-3468,
[Article]

Abstract

Lipid bilayers provide many benefits for ion channel recordings, such as control of membrane composition and transport molecules. However, they suffer from high membrane capacitance limiting the bandwidth and impeding analysis of fast gating. This can be overcome by fitting the deviations of the open channel noise from the baseline noise by extended beta distributions. We demonstrate this analysis step-by-step by applying it to the example of viral K(+)  channels (Kcv), from the choice of the gating model through the fitting process, validation of the results, and what kinds of results can be obtained. These voltage sensor-less channels show profoundly voltage-dependent gating with dwell times in the closed state of about 50 μs. Mutations assign it to the selectivity filter. This article is protected by copyright. All rights reserved.

Item Type: Article
Erschienen: 2017
Creators: Rauh, O. and Hansen, U. P. and Mach, S. and Hartel, A. J. W. and Shepard, K. L. and Thiel, Gerhard and Schroeder, Indra
Title: Extended beta distributions open the access to fast gating in bilayer experiments: assigning the voltage-dependent gating to the selectivity filter.
Language: English
Abstract:

Lipid bilayers provide many benefits for ion channel recordings, such as control of membrane composition and transport molecules. However, they suffer from high membrane capacitance limiting the bandwidth and impeding analysis of fast gating. This can be overcome by fitting the deviations of the open channel noise from the baseline noise by extended beta distributions. We demonstrate this analysis step-by-step by applying it to the example of viral K(+)  channels (Kcv), from the choice of the gating model through the fitting process, validation of the results, and what kinds of results can be obtained. These voltage sensor-less channels show profoundly voltage-dependent gating with dwell times in the closed state of about 50 μs. Mutations assign it to the selectivity filter. This article is protected by copyright. All rights reserved.

Journal or Publication Title: FEBS letters
Volume: 591
Number: 23
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 14 Nov 2017 10:33
Identification Number: pmid:29106736
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