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Vesicle fusion and fission in plants and yeast.

Cucu, Bayram and Degreif, Daniel and Bertl, Adam and Thiel, Gerhard (2017):
Vesicle fusion and fission in plants and yeast.
In: Cell calcium, 67, pp. 40-45. ISSN 1532-1991,
[Article]

Abstract

Measurements of the membrane capacitance on animal cells has provided an excellent technique for monitoring of exo- and endocytotic activity in intact living cells. Here we review recent data in which the same technique was applied to plant cells and cells of the budding yeast Saccharomyces cerevisiae. The data show that unitary exo- and endocytotic events can also be measured with the same technique after removing the cell wall from these cells. The resulting protoplasts execute the same type of transient and permanent fusion/fission that is known from animal cells. Also the size of the vesicles, which are fusing or budding, are of the same order of magnitude as those recorded in animal cells. Together these data support the view of an evolutionary conserved mechanism for unitary exo- and endocytosis events in eukaryotes. The successful recordings of exo- and endocytotic activity in Saccharomyces cerevisiae by capacitance measurements now pave the way for correlating the abundant information on the molecular machinery of exo- and endocytosis in this model organism with distinct functional properties.

Item Type: Article
Erschienen: 2017
Creators: Cucu, Bayram and Degreif, Daniel and Bertl, Adam and Thiel, Gerhard
Title: Vesicle fusion and fission in plants and yeast.
Language: English
Abstract:

Measurements of the membrane capacitance on animal cells has provided an excellent technique for monitoring of exo- and endocytotic activity in intact living cells. Here we review recent data in which the same technique was applied to plant cells and cells of the budding yeast Saccharomyces cerevisiae. The data show that unitary exo- and endocytotic events can also be measured with the same technique after removing the cell wall from these cells. The resulting protoplasts execute the same type of transient and permanent fusion/fission that is known from animal cells. Also the size of the vesicles, which are fusing or budding, are of the same order of magnitude as those recorded in animal cells. Together these data support the view of an evolutionary conserved mechanism for unitary exo- and endocytosis events in eukaryotes. The successful recordings of exo- and endocytotic activity in Saccharomyces cerevisiae by capacitance measurements now pave the way for correlating the abundant information on the molecular machinery of exo- and endocytosis in this model organism with distinct functional properties.

Journal or Publication Title: Cell calcium
Journal volume: 67
Divisions: 10 Department of Biology
10 Department of Biology > Yeast Membrane Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 24 Oct 2017 06:11
Identification Number: pmid:29029789
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