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The role of inoculum and reactor configuration for microbial community composition and dynamics in mainstream partial nitritation anammox reactors

Agrawal, Shelesh and Karst, Søren M. and Gilbert, Eva M. and Horn, Harald and Nielsen, Per H. and Lackner, Susanne (2017):
The role of inoculum and reactor configuration for microbial community composition and dynamics in mainstream partial nitritation anammox reactors.
In: MicrobiologyOpen, Wiley, 6, (2), ISSN 2045-8827,
[Online-Edition: http://doi.org/10.1002/mbo3.456],
[Article]

Abstract

Implementation of partial nitritation anammox (PNA) in the mainstream (municipal wastewater treatment) is still under investigation. Microbial community structure and reactor type can influence the performance of PNA reactor; yet, little is known about the role of the community composition of the inoculum and the reactor configuration under mainstream conditions. Therefore, this study investigated the community structure of inocula of different origin and their consecutive community dynamics in four different lab-scale PNA reactors with 16S rRNA gene amplicon sequencing. These reactors were operated for almost 1 year and subjected to realistic seasonal temperature fluctuations as in moderate climate regions, that is, from 20°C in summer to 10°C in winter. The sequencing analysis revealed that the bacterial community in the reactors comprised: (1) a nitrifying community (consisting of anaerobic ammonium-oxidizing bacteria (AnAOB), ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB)); (2) different heterotrophic denitrifying bacteria and other putative heterotrophic bacteria (HB). The nitrifying community was the same in all four reactors at the genus level, although the biomasses were of different origin. Community dynamics revealed a stable community in the moving bed biofilm reactors (MBBR) in contrast to the sequencing batch reactors (SBR) at the genus level. Moreover, the reactor design seemed to influence the community dynamics, and reactor operation significantly influenced the overall community composition. The MBBR seems to be the reactor type of choice for mainstream wastewater treatment.

Item Type: Article
Erschienen: 2017
Creators: Agrawal, Shelesh and Karst, Søren M. and Gilbert, Eva M. and Horn, Harald and Nielsen, Per H. and Lackner, Susanne
Title: The role of inoculum and reactor configuration for microbial community composition and dynamics in mainstream partial nitritation anammox reactors
Language: English
Abstract:

Implementation of partial nitritation anammox (PNA) in the mainstream (municipal wastewater treatment) is still under investigation. Microbial community structure and reactor type can influence the performance of PNA reactor; yet, little is known about the role of the community composition of the inoculum and the reactor configuration under mainstream conditions. Therefore, this study investigated the community structure of inocula of different origin and their consecutive community dynamics in four different lab-scale PNA reactors with 16S rRNA gene amplicon sequencing. These reactors were operated for almost 1 year and subjected to realistic seasonal temperature fluctuations as in moderate climate regions, that is, from 20°C in summer to 10°C in winter. The sequencing analysis revealed that the bacterial community in the reactors comprised: (1) a nitrifying community (consisting of anaerobic ammonium-oxidizing bacteria (AnAOB), ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB)); (2) different heterotrophic denitrifying bacteria and other putative heterotrophic bacteria (HB). The nitrifying community was the same in all four reactors at the genus level, although the biomasses were of different origin. Community dynamics revealed a stable community in the moving bed biofilm reactors (MBBR) in contrast to the sequencing batch reactors (SBR) at the genus level. Moreover, the reactor design seemed to influence the community dynamics, and reactor operation significantly influenced the overall community composition. The MBBR seems to be the reactor type of choice for mainstream wastewater treatment.

Journal or Publication Title: MicrobiologyOpen
Volume: 6
Number: 2
Publisher: Wiley
Uncontrolled Keywords: Biofilm Bioreactors Molecular microbial ecology Nitrogen metabolism
Divisions: 13 Department of Civil and Environmental Engineering Sciences
13 Department of Civil and Environmental Engineering Sciences > Institute IWAR
13 Department of Civil and Environmental Engineering Sciences > Institute IWAR > Wastewater Engineering
Date Deposited: 07 May 2017 19:55
Official URL: http://doi.org/10.1002/mbo3.456
URN: urn:nbn:de:tuda-tuprints-61409
Identification Number: doi:10.1002/mbo3.456
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