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Yeast-Based Screening System for the Selection of Functional Light-Driven K(+) Channels.

Cosentino, Cristian and Alberio, Laura and Thiel, Gerhard and Moroni, Anna (2017):
Yeast-Based Screening System for the Selection of Functional Light-Driven K(+) Channels.
In: Methods in molecular biology (Clifton, N.J.), pp. 271-285, 1596, ISSN 1940-6029,
[Article]

Abstract

Ion channels control the electrical properties of cells by opening and closing (gating) in response to a wide palette of environmental and physiological stimuli. Endowing ion channels with the possibility to be gated by remotely applied stimuli, such as light, provides a tool for in vivo control of cellular functions in behaving animals. We have engineered a synthetic light-gated potassium (K(+)) channel by connecting an exogenous plant photoreceptor LOV2 domain to the K(+) channel pore Kcv. Here, we describe the experimental strategy that we have used to evolve the properties of the channel toward full control of light on pore gating. Our method combines rational and random mutagenesis of the channel followed by a yeast-based screening system for light-activated K(+) conductance.

Item Type: Article
Erschienen: 2017
Creators: Cosentino, Cristian and Alberio, Laura and Thiel, Gerhard and Moroni, Anna
Title: Yeast-Based Screening System for the Selection of Functional Light-Driven K(+) Channels.
Language: English
Abstract:

Ion channels control the electrical properties of cells by opening and closing (gating) in response to a wide palette of environmental and physiological stimuli. Endowing ion channels with the possibility to be gated by remotely applied stimuli, such as light, provides a tool for in vivo control of cellular functions in behaving animals. We have engineered a synthetic light-gated potassium (K(+)) channel by connecting an exogenous plant photoreceptor LOV2 domain to the K(+) channel pore Kcv. Here, we describe the experimental strategy that we have used to evolve the properties of the channel toward full control of light on pore gating. Our method combines rational and random mutagenesis of the channel followed by a yeast-based screening system for light-activated K(+) conductance.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Volume: 1596
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 23 Mar 2017 07:59
Identification Number: pmid:28293893
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