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Visualization of the Nucleolus in Living Cells with Cell-Penetrating Fluorescent Peptides.

Martin, Robert M. and Herce, Henry D. and Ludwig, Anne K. and Cardoso, M. Cristina (2016):
Visualization of the Nucleolus in Living Cells with Cell-Penetrating Fluorescent Peptides.
1455, In: Methods in molecular biology (Clifton, N.J.), pp. 71-82, ISSN 1940-6029, [Article]

Abstract

The nucleolus is the hallmark of nuclear compartmentalization and has been shown to exert multiple roles in cellular metabolism besides its main function as the place of ribosomal RNA synthesis and assembly of ribosomes. The nucleolus plays also a major role in nuclear organization as the largest compartment within the nucleus. The prominent structure of the nucleolus can be detected using contrast light microscopy providing an approximate localization of the nucleolus, but this approach does not allow to determine accurately the three-dimensional structure of the nucleolus in cells and tissues. Immunofluorescence staining with antibodies specific to nucleolar proteins albeit very useful is time consuming, normally antibodies recognize their epitopes only within a small range of species and is applicable only in fixed cells. Here, we present a simple method to selectively and accurately label this ubiquitous subnuclear compartment in living cells of a large range of species using a fluorescently labeled cell-penetrating peptide.

Item Type: Article
Erschienen: 2016
Creators: Martin, Robert M. and Herce, Henry D. and Ludwig, Anne K. and Cardoso, M. Cristina
Title: Visualization of the Nucleolus in Living Cells with Cell-Penetrating Fluorescent Peptides.
Language: English
Abstract:

The nucleolus is the hallmark of nuclear compartmentalization and has been shown to exert multiple roles in cellular metabolism besides its main function as the place of ribosomal RNA synthesis and assembly of ribosomes. The nucleolus plays also a major role in nuclear organization as the largest compartment within the nucleus. The prominent structure of the nucleolus can be detected using contrast light microscopy providing an approximate localization of the nucleolus, but this approach does not allow to determine accurately the three-dimensional structure of the nucleolus in cells and tissues. Immunofluorescence staining with antibodies specific to nucleolar proteins albeit very useful is time consuming, normally antibodies recognize their epitopes only within a small range of species and is applicable only in fixed cells. Here, we present a simple method to selectively and accurately label this ubiquitous subnuclear compartment in living cells of a large range of species using a fluorescently labeled cell-penetrating peptide.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Volume: 1455
Divisions: 10 Department of Biology
10 Department of Biology > Cell Biology and Epigenetics
Date Deposited: 06 Sep 2016 07:39
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