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Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae.

Zhang, Ying and Schäffer, Thea and Wölfle, Tina and Fitzke, Edith and Thiel, Gerhard and Rospert, Sabine (2016):
Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae.
36, In: Molecular and cellular biology, (18), pp. 2374-2383, ISSN 1098-5549, [Article]

Abstract

Targeting of transmembrane proteins to the endoplasmic reticulum proceeds via either the SRP or the GET pathway, consisting of Get1-5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single, C-terminal transmembrane domain termed tail-anchor. Here we dissect the role of the SRP and GET pathway in the sorting of homologous, two-membrane spanning K(+) channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv was targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV was targeted posttranslationally via its C-terminal transmembrane domain, which recruited Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv did not only recruit SRP, but also the Get4-5 module of the GET pathway to ribosomes. Ribosome-binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways.

Item Type: Article
Erschienen: 2016
Creators: Zhang, Ying and Schäffer, Thea and Wölfle, Tina and Fitzke, Edith and Thiel, Gerhard and Rospert, Sabine
Title: Cotranslational intersection between the SRP and GET targeting pathways to the ER of Saccharomyces cerevisiae.
Language: English
Abstract:

Targeting of transmembrane proteins to the endoplasmic reticulum proceeds via either the SRP or the GET pathway, consisting of Get1-5 and Sgt2. While SRP cotranslationally targets membrane proteins containing one or multiple transmembrane domains, the GET pathway posttranslationally targets proteins containing a single, C-terminal transmembrane domain termed tail-anchor. Here we dissect the role of the SRP and GET pathway in the sorting of homologous, two-membrane spanning K(+) channel proteins termed Kcv, Kesv, and Kesv-VV. We show that Kcv was targeted to the ER cotranslationally via its N-terminal transmembrane domain, while Kesv-VV was targeted posttranslationally via its C-terminal transmembrane domain, which recruited Get4-5/Sgt2 and Get3. Unexpectedly, nascent Kcv did not only recruit SRP, but also the Get4-5 module of the GET pathway to ribosomes. Ribosome-binding of Get4-5 was independent of Sgt2 and was strongly outcompeted by SRP. The combined data indicate a previously unrecognized cotranslational interplay between the SRP and GET pathways.

Journal or Publication Title: Molecular and cellular biology
Volume: 36
Number: 18
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 05 Jul 2016 08:40
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