TU Darmstadt / ULB / TUbiblio

What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch.

Duchardt-Ferner, Elke and Gottstein-Schmidtke, Sina R. and Weigand, Julia E. and Ohlenschläger, Oliver and Wurm, Jan-Philip and Hammann, Christian and Suess, Beatrix and Wöhnert, Jens (2016):
What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch.
In: Angewandte Chemie (International ed. in English), pp. 1527-1530, 55, (4), ISSN 1521-3773, [Article]

Abstract

To ensure appropriate metabolic regulation, riboswitches must discriminate efficiently between their target ligands and chemically similar molecules that are also present in the cell. A remarkable example of efficient ligand discrimination is a synthetic neomycin-sensing riboswitch. Paromomycin, which differs from neomycin only by the substitution of a single amino group with a hydroxy group, also binds but does not flip the riboswitch. Interestingly, the solution structures of the two riboswitch-ligand complexes are virtually identical. In this work, we demonstrate that the local loss of key intermolecular interactions at the substitution site is translated through a defined network of intramolecular interactions into global changes in RNA conformational dynamics. The remarkable specificity of this riboswitch is thus based on structural dynamics rather than static structural differences. In this respect, the neomycin riboswitch is a model for many of its natural counterparts.

Item Type: Article
Erschienen: 2016
Creators: Duchardt-Ferner, Elke and Gottstein-Schmidtke, Sina R. and Weigand, Julia E. and Ohlenschläger, Oliver and Wurm, Jan-Philip and Hammann, Christian and Suess, Beatrix and Wöhnert, Jens
Title: What a Difference an OH Makes: Conformational Dynamics as the Basis for the Ligand Specificity of the Neomycin-Sensing Riboswitch.
Language: English
Abstract:

To ensure appropriate metabolic regulation, riboswitches must discriminate efficiently between their target ligands and chemically similar molecules that are also present in the cell. A remarkable example of efficient ligand discrimination is a synthetic neomycin-sensing riboswitch. Paromomycin, which differs from neomycin only by the substitution of a single amino group with a hydroxy group, also binds but does not flip the riboswitch. Interestingly, the solution structures of the two riboswitch-ligand complexes are virtually identical. In this work, we demonstrate that the local loss of key intermolecular interactions at the substitution site is translated through a defined network of intramolecular interactions into global changes in RNA conformational dynamics. The remarkable specificity of this riboswitch is thus based on structural dynamics rather than static structural differences. In this respect, the neomycin riboswitch is a model for many of its natural counterparts.

Journal or Publication Title: Angewandte Chemie (International ed. in English)
Volume: 55
Number: 4
Divisions: 10 Department of Biology
10 Department of Biology > Synthetic Genetic Circuits
Date Deposited: 22 Dec 2015 11:12
Export:

Optionen (nur für Redakteure)

View Item View Item