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Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry.

Vogel, Marc ; Suess, Beatrix (2016)
Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry.
In: Methods in molecular biology (Clifton, N.J.), 1380
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Isothermal titration calorimetry (ITC) is a powerful label-free technique to determine the binding constant as well as thermodynamic parameters of a binding reaction and is therefore well suited for the analysis of small molecule-RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls.

Typ des Eintrags: Artikel
Erschienen: 2016
Autor(en): Vogel, Marc ; Suess, Beatrix
Art des Eintrags: Bibliographie
Titel: Label-Free Determination of the Dissociation Constant of Small Molecule-Aptamer Interaction by Isothermal Titration Calorimetry.
Sprache: Englisch
Publikationsjahr: 2016
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Methods in molecular biology (Clifton, N.J.)
Jahrgang/Volume einer Zeitschrift: 1380
Kurzbeschreibung (Abstract):

Isothermal titration calorimetry (ITC) is a powerful label-free technique to determine the binding constant as well as thermodynamic parameters of a binding reaction and is therefore well suited for the analysis of small molecule-RNA aptamer interaction. We will introduce you to the method and present a protocol for sample preparation and the calorimetric measurement. A detailed note section will point out useful tips and pitfalls.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology")
Hinterlegungsdatum: 17 Nov 2015 11:23
Letzte Änderung: 17 Nov 2015 11:23
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