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Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase.

Schumacher, Dominik ; Helma, Jonas ; Mann, Florian A. ; Pichler, Garwin ; Natale, Francesco ; Krause, Eberhard ; Cardoso, M. Cristina ; Hackenberger, Christian P. R. ; Leonhardt, Heinrich (2015)
Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase.
In: Angewandte Chemie (International ed. in English), 54 (46)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin-derived recognition sequence (Tub-tag). This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP, and ubiquitin.

Typ des Eintrags: Artikel
Erschienen: 2015
Autor(en): Schumacher, Dominik ; Helma, Jonas ; Mann, Florian A. ; Pichler, Garwin ; Natale, Francesco ; Krause, Eberhard ; Cardoso, M. Cristina ; Hackenberger, Christian P. R. ; Leonhardt, Heinrich
Art des Eintrags: Bibliographie
Titel: Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase.
Sprache: Englisch
Publikationsjahr: 2015
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Angewandte Chemie (International ed. in English)
Jahrgang/Volume einer Zeitschrift: 54
(Heft-)Nummer: 46
Kurzbeschreibung (Abstract):

A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin-derived recognition sequence (Tub-tag). This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP, and ubiquitin.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Cell Biology and Epigenetics
Hinterlegungsdatum: 01 Okt 2015 06:29
Letzte Änderung: 22 Dez 2015 11:27
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