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Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase.

Schumacher, Dominik and Helma, Jonas and Mann, Florian A. and Pichler, Garwin and Natale, Francesco and Krause, Eberhard and Cardoso, M. Cristina and Hackenberger, Christian P. R. and Leonhardt, Heinrich (2015):
Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase.
In: Angewandte Chemie (International ed. in English), pp. 13787-13791, 54, (46), ISSN 1521-3773,
[Article]

Abstract

A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin-derived recognition sequence (Tub-tag). This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP, and ubiquitin.

Item Type: Article
Erschienen: 2015
Creators: Schumacher, Dominik and Helma, Jonas and Mann, Florian A. and Pichler, Garwin and Natale, Francesco and Krause, Eberhard and Cardoso, M. Cristina and Hackenberger, Christian P. R. and Leonhardt, Heinrich
Title: Versatile and Efficient Site-Specific Protein Functionalization by Tubulin Tyrosine Ligase.
Language: English
Abstract:

A novel chemoenzymatic approach for simple and fast site-specific protein labeling is reported. Recombinant tubulin tyrosine ligase (TTL) was repurposed to attach various unnatural tyrosine derivatives as small bioorthogonal handles to proteins containing a short tubulin-derived recognition sequence (Tub-tag). This novel strategy enables a broad range of high-yielding and fast chemoselective C-terminal protein modifications on isolated proteins or in cell lysates for applications in biochemistry, cell biology, and beyond, as demonstrated by the site-specific labeling of nanobodies, GFP, and ubiquitin.

Journal or Publication Title: Angewandte Chemie (International ed. in English)
Volume: 54
Number: 46
Divisions: 10 Department of Biology
10 Department of Biology > Cell Biology and Epigenetics
Date Deposited: 01 Oct 2015 06:29
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