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RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.

Bi, Zhen and Merl-Pham, Juliane and Uehlein, Norbert and Zimmer, Ina and Mühlhans, Stefanie and Aichler, Michaela and Walch, Axel Karl and Kaldenhoff, Ralf and Palme, Klaus and Schnitzler, Jörg-Peter and Block, Katja (2015):
RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.
In: Journal of proteomics, pp. 321-332, 128, ISSN 1876-7737,
[Article]

Abstract

Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensive leaf plasma membrane proteome and physiome analysis. First, inhibition of PIP synthesis strongly altered the leaf plasma membrane protein composition. Strikingly, several signaling components and transporters involved in the regulation of stomatal movement were differentially regulated in transgenic poplars. Furthermore, hormonal crosstalk related to abscisic acid, auxin and brassinosteroids was altered, in addition to cell wall biosynthesis/cutinization, the organization of cellular structures and membrane trafficking. A physiological analysis confirmed the proteomic results. The leaves had wider opened stomata and higher net CO2 assimilation and transpiration rates as well as greater mesophyll conductance for CO2 (gm) and leaf hydraulic conductance (Kleaf). Based on these results, we conclude that PIP proteins not only play essential roles in whole leaf water and CO2 flux but have important roles in the regulation of stomatal movement. Biological significance The present work is a comprehensive survey combining leaf plasma membrane proteomics and physiological methods to assess the functionality of the whole PIP subfamily in tree model system.

Item Type: Article
Erschienen: 2015
Creators: Bi, Zhen and Merl-Pham, Juliane and Uehlein, Norbert and Zimmer, Ina and Mühlhans, Stefanie and Aichler, Michaela and Walch, Axel Karl and Kaldenhoff, Ralf and Palme, Klaus and Schnitzler, Jörg-Peter and Block, Katja
Title: RNAi-mediated downregulation of poplar plasma membrane intrinsic proteins (PIPs) changes plasma membrane proteome composition and affects leaf physiology.
Language: English
Abstract:

Plasma membrane intrinsic proteins (PIPs) are one subfamily of aquaporins that mediate the transmembrane transport of water. To reveal their function in poplar, we generated transgenic poplar plants in which the translation of PIP genes was downregulated by RNA interference investigated these plants with a comprehensive leaf plasma membrane proteome and physiome analysis. First, inhibition of PIP synthesis strongly altered the leaf plasma membrane protein composition. Strikingly, several signaling components and transporters involved in the regulation of stomatal movement were differentially regulated in transgenic poplars. Furthermore, hormonal crosstalk related to abscisic acid, auxin and brassinosteroids was altered, in addition to cell wall biosynthesis/cutinization, the organization of cellular structures and membrane trafficking. A physiological analysis confirmed the proteomic results. The leaves had wider opened stomata and higher net CO2 assimilation and transpiration rates as well as greater mesophyll conductance for CO2 (gm) and leaf hydraulic conductance (Kleaf). Based on these results, we conclude that PIP proteins not only play essential roles in whole leaf water and CO2 flux but have important roles in the regulation of stomatal movement. Biological significance The present work is a comprehensive survey combining leaf plasma membrane proteomics and physiological methods to assess the functionality of the whole PIP subfamily in tree model system.

Journal or Publication Title: Journal of proteomics
Volume: 128
Divisions: 10 Department of Biology
10 Department of Biology > Applied Plant Sciences
Date Deposited: 01 Sep 2015 10:33
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