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Optogenetics. Engineering of a light-gated potassium channel.

Cosentino, Cristian and Alberio, Laura and Gazzarrini, Sabrina and Aquila, Marco and Romano, Edoardo and Cermenati, Solei and Zuccolini, Paolo and Petersen, Jan and Beltrame, Monica and Van Etten, James L. and Christie, John M. and Thiel, Gerhard and Moroni, Anna (2015):
Optogenetics. Engineering of a light-gated potassium channel.
In: Science (New York, N.Y.), pp. 707-10, 348, (6235), ISSN 1095-9203,
[Article]

Abstract

The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K(+)) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K(+) channel 1 (BLINK1) engineered by fusing the plant LOV2-Jα photosensory module to the small viral K(+) channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K(+) equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.

Item Type: Article
Erschienen: 2015
Creators: Cosentino, Cristian and Alberio, Laura and Gazzarrini, Sabrina and Aquila, Marco and Romano, Edoardo and Cermenati, Solei and Zuccolini, Paolo and Petersen, Jan and Beltrame, Monica and Van Etten, James L. and Christie, John M. and Thiel, Gerhard and Moroni, Anna
Title: Optogenetics. Engineering of a light-gated potassium channel.
Language: German
Abstract:

The present palette of opsin-based optogenetic tools lacks a light-gated potassium (K(+)) channel desirable for silencing of excitable cells. Here, we describe the construction of a blue-light-induced K(+) channel 1 (BLINK1) engineered by fusing the plant LOV2-Jα photosensory module to the small viral K(+) channel Kcv. BLINK1 exhibits biophysical features of Kcv, including K(+) selectivity and high single-channel conductance but reversibly photoactivates in blue light. Opening of BLINK1 channels hyperpolarizes the cell to the K(+) equilibrium potential. Ectopic expression of BLINK1 reversibly inhibits the escape response in light-exposed zebrafish larvae. BLINK1 therefore provides a single-component optogenetic tool that can establish prolonged, physiological hyperpolarization of cells at low light intensities.

Journal or Publication Title: Science (New York, N.Y.)
Volume: 348
Number: 6235
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 29 Jun 2015 12:44
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