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Electrophysiologic and cellular characteristics of cardiomyocytes after X-ray irradiation.

Frieß, Johannes L. and Heselich, Anja and Ritter, Sylvia and Haber, Angelina and Kaiser, Nicole and Layer, Paul G. and Thielemann, Christiane (2015):
Electrophysiologic and cellular characteristics of cardiomyocytes after X-ray irradiation.
777, In: Mutation research, pp. 1-10, ISSN 1873-135X, [Article]

Abstract

The aim of this study was to investigate possible effects of ionizing irradiation on the electrophysiological functionality of cardiac myocytes in vitro. Primary chicken cardiomyocytes with spontaneous beating activity were irradiated with X-rays (dose range of 0.5-7Gy). Functional alterations of cardiac cell cultures were evaluated up to 7 days after irradiation using microelectrode arrays. As examined endpoints, cell proliferation, apoptosis, reactive oxygen species (ROS) and DNA damage were evaluated. The beat rate of the cardiac networks increased in a dose-dependent manner over one week. The duration of single action potentials was slightly shortened. Additionally, we observed lower numbers of mitotic and S-phase cells at certain time points after irradiation. Also, the number of cells with γH2AX foci increased as a function of the dose. No significant changes in the level of ROS were detected. Induction of apoptosis was generally negligibly low. This is the first report to directly show alterations in cardiac electrophysiology caused by ionizing radiation, which were detectable up to one week after irradiation.

Item Type: Article
Erschienen: 2015
Creators: Frieß, Johannes L. and Heselich, Anja and Ritter, Sylvia and Haber, Angelina and Kaiser, Nicole and Layer, Paul G. and Thielemann, Christiane
Title: Electrophysiologic and cellular characteristics of cardiomyocytes after X-ray irradiation.
Language: English
Abstract:

The aim of this study was to investigate possible effects of ionizing irradiation on the electrophysiological functionality of cardiac myocytes in vitro. Primary chicken cardiomyocytes with spontaneous beating activity were irradiated with X-rays (dose range of 0.5-7Gy). Functional alterations of cardiac cell cultures were evaluated up to 7 days after irradiation using microelectrode arrays. As examined endpoints, cell proliferation, apoptosis, reactive oxygen species (ROS) and DNA damage were evaluated. The beat rate of the cardiac networks increased in a dose-dependent manner over one week. The duration of single action potentials was slightly shortened. Additionally, we observed lower numbers of mitotic and S-phase cells at certain time points after irradiation. Also, the number of cells with γH2AX foci increased as a function of the dose. No significant changes in the level of ROS were detected. Induction of apoptosis was generally negligibly low. This is the first report to directly show alterations in cardiac electrophysiology caused by ionizing radiation, which were detectable up to one week after irradiation.

Journal or Publication Title: Mutation research
Volume: 777
Divisions: 10 Department of Biology
10 Department of Biology > Developmental Biology and Neurogenetics
Date Deposited: 06 May 2015 06:25
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