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Tetracycline determines the conformation of its aptamer at physiological magnesium concentrations.

Reuss, Andreas J. ; Vogel, Marc ; Weigand, Julia E. ; Suess, Beatrix ; Wachtveitl, Josef (2014)
Tetracycline determines the conformation of its aptamer at physiological magnesium concentrations.
In: Biophysical journal, 107 (12)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Synthetic riboswitches are versatile tools for the study and manipulation of biological systems. Yet, the underlying mechanisms governing its structural properties and regulation under physiological conditions are poorly studied. We performed spectroscopic and calorimetric experiments to explore the folding kinetics and thermodynamics of the tetracycline-binding aptamer, which can be employed as synthetic riboswitch, in the range of physiological magnesium concentrations. The dissociation constant of the ligand-aptamer complex was found to strongly depend on the magnesium concentration. At physiological magnesium concentrations, tetracycline induces a significant conformational shift from a compact, but heterogeneous intermediate state toward the completely formed set of tertiary interactions defining the regulation-competent structure. Thus, the switching functionality of the tetracycline-binding aptamer appears to include both a conformational rearrangement toward the regulation-competent structure and its thermodynamic stabilization.

Typ des Eintrags: Artikel
Erschienen: 2014
Autor(en): Reuss, Andreas J. ; Vogel, Marc ; Weigand, Julia E. ; Suess, Beatrix ; Wachtveitl, Josef
Art des Eintrags: Bibliographie
Titel: Tetracycline determines the conformation of its aptamer at physiological magnesium concentrations.
Sprache: Englisch
Publikationsjahr: 2014
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Biophysical journal
Jahrgang/Volume einer Zeitschrift: 107
(Heft-)Nummer: 12
Kurzbeschreibung (Abstract):

Synthetic riboswitches are versatile tools for the study and manipulation of biological systems. Yet, the underlying mechanisms governing its structural properties and regulation under physiological conditions are poorly studied. We performed spectroscopic and calorimetric experiments to explore the folding kinetics and thermodynamics of the tetracycline-binding aptamer, which can be employed as synthetic riboswitch, in the range of physiological magnesium concentrations. The dissociation constant of the ligand-aptamer complex was found to strongly depend on the magnesium concentration. At physiological magnesium concentrations, tetracycline induces a significant conformational shift from a compact, but heterogeneous intermediate state toward the completely formed set of tertiary interactions defining the regulation-competent structure. Thus, the switching functionality of the tetracycline-binding aptamer appears to include both a conformational rearrangement toward the regulation-competent structure and its thermodynamic stabilization.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology")
10 Fachbereich Biologie > RNA Biochemie
Hinterlegungsdatum: 23 Dez 2014 08:45
Letzte Änderung: 06 Nov 2020 07:14
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