TU Darmstadt / ULB / TUbiblio

Live-cell targeting of His-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes.

Wieneke, Ralph ; Laboria, Noemi ; Rajan, Malini ; Kollmannsperger, Alina ; Natale, Francesco ; Cardoso, M. Cristina ; Tampé, Robert (2014)
Live-cell targeting of His-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes.
In: Journal of the American Chemical Society, 136 (40)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

The selective and fast labeling of proteins in living cells is a major challenge. Live-cell labeling techniques require a high specificity, high labeling density, and cell permeability of the tagging molecule to target the protein of interest. Here, we report on the site-specific, rapid and efficient labeling of endogenous and recombinant histidine-tagged proteins at distinct subcellular compartments using cell-penetrating multivalent chelator carrier complexes. In vivo labeling was followed in real-time in living cells, demonstrating a high specificity and high degree of colocalization in the crowded cellular environment.

Typ des Eintrags: Artikel
Erschienen: 2014
Autor(en): Wieneke, Ralph ; Laboria, Noemi ; Rajan, Malini ; Kollmannsperger, Alina ; Natale, Francesco ; Cardoso, M. Cristina ; Tampé, Robert
Art des Eintrags: Bibliographie
Titel: Live-cell targeting of His-tagged proteins by multivalent N-nitrilotriacetic acid carrier complexes.
Sprache: Englisch
Publikationsjahr: 2014
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Journal of the American Chemical Society
Jahrgang/Volume einer Zeitschrift: 136
(Heft-)Nummer: 40
Kurzbeschreibung (Abstract):

The selective and fast labeling of proteins in living cells is a major challenge. Live-cell labeling techniques require a high specificity, high labeling density, and cell permeability of the tagging molecule to target the protein of interest. Here, we report on the site-specific, rapid and efficient labeling of endogenous and recombinant histidine-tagged proteins at distinct subcellular compartments using cell-penetrating multivalent chelator carrier complexes. In vivo labeling was followed in real-time in living cells, demonstrating a high specificity and high degree of colocalization in the crowded cellular environment.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Cell Biology and Epigenetics
Hinterlegungsdatum: 13 Nov 2014 08:05
Letzte Änderung: 13 Nov 2014 08:05
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Frage zum Eintrag Frage zum Eintrag

Optionen (nur für Redakteure)
Redaktionelle Details anzeigen Redaktionelle Details anzeigen