TU Darmstadt / ULB / TUbiblio

Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis.

Agarkova, Irina and Hertel, Brigitte and Zhang, Xinzheng and Lane, Les and Tchourbanov, Alexander and Dunigan, David D. and Thiel, Gerhard and Rossmann, Michael G. and Van Etten, James L. :
Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis.
In: Virology (466-467) pp. 95-102. ISSN 1096-0341
[Article] , (2014)

Abstract

Chloroviruses infect their hosts by specifically binding to and degrading the cell wall of their algal hosts at the site of attachment, using an intrinsic digesting enzyme(s). Chlorovirus PBCV-1 stored as a lysate survived longer than virus alone, suggesting virus attachment to cellular debris may be reversible. Ghost cells (algal cells extracted with methanol) were used as a model to study reversibility of PBCV-1 attachment because ghost cells are as susceptible to attachment and wall digestion as are live cells. Reversibility of attachment to ghost cells was examined by releasing attached virions with a cell wall degrading enzyme extract. The majority of the released virions retained infectivity even after re-incubating the released virions with ghost cells two times. Thus the chloroviruses appear to have a dynamic attachment strategy that may be beneficial in indigenous environments where cell wall debris can act as a refuge until appropriate host cells are available.

Item Type: Article
Erschienen: 2014
Creators: Agarkova, Irina and Hertel, Brigitte and Zhang, Xinzheng and Lane, Les and Tchourbanov, Alexander and Dunigan, David D. and Thiel, Gerhard and Rossmann, Michael G. and Van Etten, James L.
Title: Dynamic attachment of Chlorovirus PBCV-1 to Chlorella variabilis.
Language: English
Abstract:

Chloroviruses infect their hosts by specifically binding to and degrading the cell wall of their algal hosts at the site of attachment, using an intrinsic digesting enzyme(s). Chlorovirus PBCV-1 stored as a lysate survived longer than virus alone, suggesting virus attachment to cellular debris may be reversible. Ghost cells (algal cells extracted with methanol) were used as a model to study reversibility of PBCV-1 attachment because ghost cells are as susceptible to attachment and wall digestion as are live cells. Reversibility of attachment to ghost cells was examined by releasing attached virions with a cell wall degrading enzyme extract. The majority of the released virions retained infectivity even after re-incubating the released virions with ghost cells two times. Thus the chloroviruses appear to have a dynamic attachment strategy that may be beneficial in indigenous environments where cell wall debris can act as a refuge until appropriate host cells are available.

Journal or Publication Title: Virology
Number: 466-467
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 30 Sep 2014 09:29
Export:

Optionen (nur für Redakteure)

View Item View Item