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Influence of repair proteins and chromatin modifiers on mobility of DNA double-strand breaks induced by heavy ion irradiation

Becker, Linda Carmen Annabelle :
Influence of repair proteins and chromatin modifiers on mobility of DNA double-strand breaks induced by heavy ion irradiation.
[Online-Edition: http://tuprints.ulb.tu-darmstadt.de/4038]
TU Darmstadt , Darmstadt
[Ph.D. Thesis], (2014)

Official URL: http://tuprints.ulb.tu-darmstadt.de/4038

Abstract

Ionizing radiation induces DNA double strand breaks (DSBs) in cell nuclei which represent the most dangerous type of DNA lesions. Through error prone repair mechanisms they can lead to the formation of chromosome rearrangements, favoring genomic instability and the development of cancer. Movement of DSBs and surrounding chromatin domains is expected to influence DSB repair and promote the formation of chromosomal translocations. Addressing this expectation, DSB mobility was analyzed after heavy ion irradiation. To gain insight into influences of chromatin organization and repair related factors on the dynamic behavior of DSB sites, knockdown or inhibition of repair proteins and chromatin modifying proteins was used. Occurring changes in irradiation induced foci (IRIF) mobility were examined either by immunofluorescent analysis of fixed samples or by live cell microscopy.

Item Type: Ph.D. Thesis
Erschienen: 2014
Creators: Becker, Linda Carmen Annabelle
Title: Influence of repair proteins and chromatin modifiers on mobility of DNA double-strand breaks induced by heavy ion irradiation
Language: English
Abstract:

Ionizing radiation induces DNA double strand breaks (DSBs) in cell nuclei which represent the most dangerous type of DNA lesions. Through error prone repair mechanisms they can lead to the formation of chromosome rearrangements, favoring genomic instability and the development of cancer. Movement of DSBs and surrounding chromatin domains is expected to influence DSB repair and promote the formation of chromosomal translocations. Addressing this expectation, DSB mobility was analyzed after heavy ion irradiation. To gain insight into influences of chromatin organization and repair related factors on the dynamic behavior of DSB sites, knockdown or inhibition of repair proteins and chromatin modifying proteins was used. Occurring changes in irradiation induced foci (IRIF) mobility were examined either by immunofluorescent analysis of fixed samples or by live cell microscopy.

Place of Publication: Darmstadt
Divisions: 10 Department of Biology
10 Department of Biology > Radiation Biology and DNA Repair
Date Deposited: 17 Aug 2014 19:55
Official URL: http://tuprints.ulb.tu-darmstadt.de/4038
URN: urn:nbn:de:tuda-tuprints-40382
Referees: Durante, Prof Marco and Thiel, Prof Gerhard
Refereed / Verteidigung / mdl. Prüfung: 16 May 2014
Alternative Abstract:
Alternative abstract Language
Durch ionisierende Strahlung entstehen DNA Doppelstrangbrüche (DSB) im Zellkern, die den gefährlichsten Typ von DNA Schäden darstellen. Durch fehlerbehaftete Reparaturmechanismen können sie zu Chromosomenaustauschen führen, die genomische Instabilität und die Entstehung von Krebs zur Folge haben können. Es wird angenommen, dass die Bewegung der DSB und der darum liegenden Chromatindomänen die Reparatur beeinflusst und die Entstehung von chromosomalen Translokationen begünstigt. Um diese Vermutung zu überprüfen wurde die Mobilität von DSB nach Schwerionenbestrahlung untersucht. Zur Analyse, wie Reparaturproteine oder die Struktur von Chromatin sich auf die Bewegung von DSB auswirken, wurden Reparaturproteine und Chromatin modifizierende Proteine genetisch herunterreguliert oder inhibiert. Resultierende Änderungen der DSB Bewegung wurden durch Immunfluoreszenz Analysen in fixierten Zellen und durch Lebendzellmikroskopie bestimmt.German
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