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Pseudo painting/air bubble technique for planar lipid bilayers.

Braun, Christian J. and Baer, Tom and Moroni, Anna and Thiel, Gerhard (2014):
Pseudo painting/air bubble technique for planar lipid bilayers.
In: Journal of neuroscience methods, pp. 13-17, 233C, ISSN 1872-678X,
[Article]

Abstract

BACKGROUND

A functional reconstitution of channel proteins in planar lipid bilayers is still very versatile to study structure/function correlates under well-defined conditions at the single protein level.

NEW METHOD

In this study we present an improved planar lipid bilayer technique in which an air bubble is used for stabilizing unstable/leaky bilayers or for removing excess lipids. The bubble can also be used as a tool for reducing the number of channels in the bilayer with the goal of having only one active channel in the membrane.

RESULTS

Stable planar lipid bilayers are formed within seconds to minutes. In the case of multiple channel insertion the air bubble can be used to reduce the number of channels within minutes.

COMPARISON WITH EXISTING METHOD(S)

The simple improvement of the classical folding technique guarantees a very fast creation of stable bilayers even with difficult phospholipids in a conventional vertical bilayer set-up; it requires no modifications of the existing set-up.

CONCLUSIONS

This technique is very easy to handle and guarantees successful single channel recordings for any kind of planar lipid bilayer experiment.

Item Type: Article
Erschienen: 2014
Creators: Braun, Christian J. and Baer, Tom and Moroni, Anna and Thiel, Gerhard
Title: Pseudo painting/air bubble technique for planar lipid bilayers.
Language: English
Abstract:

BACKGROUND

A functional reconstitution of channel proteins in planar lipid bilayers is still very versatile to study structure/function correlates under well-defined conditions at the single protein level.

NEW METHOD

In this study we present an improved planar lipid bilayer technique in which an air bubble is used for stabilizing unstable/leaky bilayers or for removing excess lipids. The bubble can also be used as a tool for reducing the number of channels in the bilayer with the goal of having only one active channel in the membrane.

RESULTS

Stable planar lipid bilayers are formed within seconds to minutes. In the case of multiple channel insertion the air bubble can be used to reduce the number of channels within minutes.

COMPARISON WITH EXISTING METHOD(S)

The simple improvement of the classical folding technique guarantees a very fast creation of stable bilayers even with difficult phospholipids in a conventional vertical bilayer set-up; it requires no modifications of the existing set-up.

CONCLUSIONS

This technique is very easy to handle and guarantees successful single channel recordings for any kind of planar lipid bilayer experiment.

Journal or Publication Title: Journal of neuroscience methods
Volume: 233C
Divisions: 10 Department of Biology
10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 24 Jun 2014 11:48
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