Heinrich, Eva-Marie ; Wagner, Jasmin ; Krüger, Marcus ; John, David ; Uchida, Shizuka ; Weigand, Julia E. ; Suess, Beatrix ; Dimmeler, Stefanie (2013)
Regulation of miR-17-92a cluster processing by the microRNA binding protein SND1.
In: FEBS letters, 587 (15)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
MicroRNAs are small non-coding RNAs that regulate gene expression. Although all seven members of the miR-17-92a cluster originate from one primary transcript they are differentially expressed suggesting the presence of posttranscriptional regulation. By RNA pulldown and mass spectrometry we identified SND1, a known regulator of edited RNAs, interacting with pre-miR-92a and all mature miR-17-92a members. Hypoxic conditions lead to an elevation of the pri-miR-17-92a transcript and significantly increased levels of the precursors whereas the mature miRs were not significantly changed. SND1 silencing resolved this block in processing and induced an increase in mature miRs. Together, SND1 might be the missing link between hypoxia and the differential regulation of miRNA processing.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2013 |
| Autor(en): | Heinrich, Eva-Marie ; Wagner, Jasmin ; Krüger, Marcus ; John, David ; Uchida, Shizuka ; Weigand, Julia E. ; Suess, Beatrix ; Dimmeler, Stefanie |
| Art des Eintrags: | Bibliographie |
| Titel: | Regulation of miR-17-92a cluster processing by the microRNA binding protein SND1. |
| Sprache: | Englisch |
| Publikationsjahr: | 2013 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | FEBS letters |
| Jahrgang/Volume einer Zeitschrift: | 587 |
| (Heft-)Nummer: | 15 |
| Kurzbeschreibung (Abstract): | MicroRNAs are small non-coding RNAs that regulate gene expression. Although all seven members of the miR-17-92a cluster originate from one primary transcript they are differentially expressed suggesting the presence of posttranscriptional regulation. By RNA pulldown and mass spectrometry we identified SND1, a known regulator of edited RNAs, interacting with pre-miR-92a and all mature miR-17-92a members. Hypoxic conditions lead to an elevation of the pri-miR-17-92a transcript and significantly increased levels of the precursors whereas the mature miRs were not significantly changed. SND1 silencing resolved this block in processing and induced an increase in mature miRs. Together, SND1 might be the missing link between hypoxia and the differential regulation of miRNA processing. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology") 10 Fachbereich Biologie > RNA Biochemie |
| Hinterlegungsdatum: | 03 Sep 2013 11:24 |
| Letzte Änderung: | 06 Nov 2020 07:15 |
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