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Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor.

Rudolph, Martin Michael ; Vockenhuber, Michael-Paul ; Suess, Beatrix (2013)
Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor.
In: Microbiology (Reading, England), 159
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

We have demonstrated the portability of theophylline-dependent synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor. The riboswitches mediate dose-dependent, up to 260-fold activation of reporter gene expression. The riboswitch regulation offers a simple method since only a sequence of ~85 nucleotides has to be inserted between a transcriptional start site and the start codon and no further auxiliary factors are necessary. The promoters galP2, ermEp1 and SF14 worked well in concert with the riboswitches. They allowed theophylline-dependent expression of the heterologous β-glucuronidase reporter gene but also of an endogenous gene for the agarase dagA. The successful combination of all tested promoters with the riboswitches underlines the orthogonality of riboswitch regulation. We anticipate that any further natural or synthetic promoter can be combined with the riboswitch.

Typ des Eintrags: Artikel
Erschienen: 2013
Autor(en): Rudolph, Martin Michael ; Vockenhuber, Michael-Paul ; Suess, Beatrix
Art des Eintrags: Bibliographie
Titel: Synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor.
Sprache: Englisch
Publikationsjahr: 2013
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Microbiology (Reading, England)
Jahrgang/Volume einer Zeitschrift: 159
Kurzbeschreibung (Abstract):

We have demonstrated the portability of theophylline-dependent synthetic riboswitches for the conditional control of gene expression in Streptomyces coelicolor. The riboswitches mediate dose-dependent, up to 260-fold activation of reporter gene expression. The riboswitch regulation offers a simple method since only a sequence of ~85 nucleotides has to be inserted between a transcriptional start site and the start codon and no further auxiliary factors are necessary. The promoters galP2, ermEp1 and SF14 worked well in concert with the riboswitches. They allowed theophylline-dependent expression of the heterologous β-glucuronidase reporter gene but also of an endogenous gene for the agarase dagA. The successful combination of all tested promoters with the riboswitches underlines the orthogonality of riboswitch regulation. We anticipate that any further natural or synthetic promoter can be combined with the riboswitch.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Synthetic Genetic Circuits (2020 umbenannt in "Synthetic RNA biology")
Hinterlegungsdatum: 21 Mai 2013 08:55
Letzte Änderung: 10 Sep 2013 11:37
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