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RNA helicases involved in U-insertion/deletion-type RNA editing.

Kruse, Elisabeth and Voigt, Christin and Leeder, W.-Matthias and Göringer, H. Ulrich (2013):
RNA helicases involved in U-insertion/deletion-type RNA editing.
In: Biochimica et biophysica acta - Gene regulation mechanisms, pp. 835-841, 1829, (8), ISSN 0006-3002, [Article]

Abstract

Mitochondrial pre-messenger RNAs in kinetoplastid protozoa such as the disease-causing African trypanosomes are substrates of a unique RNA editing reaction. The process is characterized by the site-specific insertion and deletion of exclusively U nucleotides and converts nonfunctional pre-mRNAs into translatable transcripts. Similar to other RNA-based metabolic pathways, RNA editing is catalyzed by a macromolecular protein complex, the editosome. Editosomes provide a reactive surface for the individual steps of the catalytic cycle and involve as key players a specific class of small, non-coding RNAs termed guide (g)RNAs. gRNAs basepair proximal to an editing site and act as quasi templates in the U-insertion/deletion reaction. Next to the editosome several accessory proteins and complexes have been identified, which contribute to different steps of the reaction. This includes matchmaking-type RNA/RNA annealing factors as well as RNA helicases of the archetypical DEAD- and DExH/D-box families. Here we summarize the current structural, genetic and biochemical knowledge of the two characterized "editing RNA helicases" and provide an outlook onto dynamic processes within the editing reaction cycle. This article is part of a Special Issue entitled: The Biology of RNA helicases - Modulation for Life.

Item Type: Article
Erschienen: 2013
Creators: Kruse, Elisabeth and Voigt, Christin and Leeder, W.-Matthias and Göringer, H. Ulrich
Title: RNA helicases involved in U-insertion/deletion-type RNA editing.
Language: English
Abstract:

Mitochondrial pre-messenger RNAs in kinetoplastid protozoa such as the disease-causing African trypanosomes are substrates of a unique RNA editing reaction. The process is characterized by the site-specific insertion and deletion of exclusively U nucleotides and converts nonfunctional pre-mRNAs into translatable transcripts. Similar to other RNA-based metabolic pathways, RNA editing is catalyzed by a macromolecular protein complex, the editosome. Editosomes provide a reactive surface for the individual steps of the catalytic cycle and involve as key players a specific class of small, non-coding RNAs termed guide (g)RNAs. gRNAs basepair proximal to an editing site and act as quasi templates in the U-insertion/deletion reaction. Next to the editosome several accessory proteins and complexes have been identified, which contribute to different steps of the reaction. This includes matchmaking-type RNA/RNA annealing factors as well as RNA helicases of the archetypical DEAD- and DExH/D-box families. Here we summarize the current structural, genetic and biochemical knowledge of the two characterized "editing RNA helicases" and provide an outlook onto dynamic processes within the editing reaction cycle. This article is part of a Special Issue entitled: The Biology of RNA helicases - Modulation for Life.

Journal or Publication Title: Biochimica et biophysica acta - Gene regulation mechanisms
Volume: 1829
Number: 8
Divisions: 10 Department of Biology
10 Department of Biology > Postranscriptional Gene Regulation and RNA Therapeutics
Date Deposited: 23 Apr 2013 09:30
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