Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells.

Hörner, Sebastian and Fabritz, Sebastian and Herce, Henry D. and Avrutina, Olga and Dietz, Christian and Stark, Robert W. and Cardoso, M. Cristina and Kolmar, Harald :
Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells.
In: Organic & biomolecular chemistry, 11 (14) pp. 2258-2265. ISSN 1477-0539
[Article] , (2013)

Abstract

Cube octameric silsesquioxanes (COSS) are among the smallest nanoparticles known to date with a diameter of only 0.7 nm. We describe a COSS-based delivery system which allows for the drug targeting in human cells. It comprises a siloxane core with seven pendant aminopropyl groups and a fluorescently labeled peptidic ligand attached to one cage corner via a reversible disulfide bond to ensure its intracellular release. Bimodal amplitude-modulated atomic force microscopy (AFM) experiments revealed the formation of dendritic COSS structures by a self-assembly of single particles on negatively charged surfaces. Nuclear targeting was demonstrated in HeLa cells by selective binding of released p21(Cip1/Waf1)-derived cargo peptide to PCNA, a protein involved in DNA replication and repair.

Item Type:	Article
Erschienen:	2013
Creators:	Hörner, Sebastian and Fabritz, Sebastian and Herce, Henry D. and Avrutina, Olga and Dietz, Christian and Stark, Robert W. and Cardoso, M. Cristina and Kolmar, Harald
Title:	Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells.
Language:	English
Abstract:	Cube octameric silsesquioxanes (COSS) are among the smallest nanoparticles known to date with a diameter of only 0.7 nm. We describe a COSS-based delivery system which allows for the drug targeting in human cells. It comprises a siloxane core with seven pendant aminopropyl groups and a fluorescently labeled peptidic ligand attached to one cage corner via a reversible disulfide bond to ensure its intracellular release. Bimodal amplitude-modulated atomic force microscopy (AFM) experiments revealed the formation of dendritic COSS structures by a self-assembly of single particles on negatively charged surfaces. Nuclear targeting was demonstrated in HeLa cells by selective binding of released p21(Cip1/Waf1)-derived cargo peptide to PCNA, a protein involved in DNA replication and repair.
Journal or Publication Title:	Organic & biomolecular chemistry
Volume:	11
Number:	14
Divisions:	10 Department of Biology ?? fb10_zoologie ?? 10 Department of Biology > Cell Biology and Epigenetics 11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces Zentrale Einrichtungen Exzellenzinitiative > Clusters of Excellence > Center of Smart Interfaces (CSI) 11 Department of Materials and Earth Sciences > Material Science Exzellenzinitiative > Clusters of Excellence 11 Department of Materials and Earth Sciences Exzellenzinitiative
Date Deposited:	09 Jan 2013 10:28
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