Hörner, Sebastian ; Fabritz, Sebastian ; Herce, Henry D. ; Avrutina, Olga ; Dietz, Christian ; Stark, Robert W. ; Cardoso, M. Cristina ; Kolmar, Harald (2013)
Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells.
In: Organic & biomolecular chemistry, 11 (14)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Cube octameric silsesquioxanes (COSS) are among the smallest nanoparticles known to date with a diameter of only 0.7 nm. We describe a COSS-based delivery system which allows for the drug targeting in human cells. It comprises a siloxane core with seven pendant aminopropyl groups and a fluorescently labeled peptidic ligand attached to one cage corner via a reversible disulfide bond to ensure its intracellular release. Bimodal amplitude-modulated atomic force microscopy (AFM) experiments revealed the formation of dendritic COSS structures by a self-assembly of single particles on negatively charged surfaces. Nuclear targeting was demonstrated in HeLa cells by selective binding of released p21(Cip1/Waf1)-derived cargo peptide to PCNA, a protein involved in DNA replication and repair.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2013 |
| Autor(en): | Hörner, Sebastian ; Fabritz, Sebastian ; Herce, Henry D. ; Avrutina, Olga ; Dietz, Christian ; Stark, Robert W. ; Cardoso, M. Cristina ; Kolmar, Harald |
| Art des Eintrags: | Bibliographie |
| Titel: | Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells. |
| Sprache: | Englisch |
| Publikationsjahr: | 2013 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Organic & biomolecular chemistry |
| Jahrgang/Volume einer Zeitschrift: | 11 |
| (Heft-)Nummer: | 14 |
| Kurzbeschreibung (Abstract): | Cube octameric silsesquioxanes (COSS) are among the smallest nanoparticles known to date with a diameter of only 0.7 nm. We describe a COSS-based delivery system which allows for the drug targeting in human cells. It comprises a siloxane core with seven pendant aminopropyl groups and a fluorescently labeled peptidic ligand attached to one cage corner via a reversible disulfide bond to ensure its intracellular release. Bimodal amplitude-modulated atomic force microscopy (AFM) experiments revealed the formation of dendritic COSS structures by a self-assembly of single particles on negatively charged surfaces. Nuclear targeting was demonstrated in HeLa cells by selective binding of released p21(Cip1/Waf1)-derived cargo peptide to PCNA, a protein involved in DNA replication and repair. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Cell Biology and Epigenetics 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Physics of Surfaces Zentrale Einrichtungen Exzellenzinitiative > Exzellenzcluster > Center of Smart Interfaces (CSI) 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft Exzellenzinitiative > Exzellenzcluster 11 Fachbereich Material- und Geowissenschaften Exzellenzinitiative |
| Hinterlegungsdatum: | 09 Jan 2013 10:28 |
| Letzte Änderung: | 26 Jul 2016 13:26 |
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