Hörner, Sebastian and Fabritz, Sebastian and Herce, Henry D. and Avrutina, Olga and Dietz, Christian and Stark, Robert W. and Cardoso, M. Cristina and Kolmar, Harald (2013):
Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells.
In: Organic & biomolecular chemistry, pp. 2258-2265, 11, (14), ISSN 1477-0539, [Article]
Abstract
Cube octameric silsesquioxanes (COSS) are among the smallest nanoparticles known to date with a diameter of only 0.7 nm. We describe a COSS-based delivery system which allows for the drug targeting in human cells. It comprises a siloxane core with seven pendant aminopropyl groups and a fluorescently labeled peptidic ligand attached to one cage corner via a reversible disulfide bond to ensure its intracellular release. Bimodal amplitude-modulated atomic force microscopy (AFM) experiments revealed the formation of dendritic COSS structures by a self-assembly of single particles on negatively charged surfaces. Nuclear targeting was demonstrated in HeLa cells by selective binding of released p21(Cip1/Waf1)-derived cargo peptide to PCNA, a protein involved in DNA replication and repair.
| Item Type: | Article |
|---|---|
| Erschienen: | 2013 |
| Creators: | Hörner, Sebastian and Fabritz, Sebastian and Herce, Henry D. and Avrutina, Olga and Dietz, Christian and Stark, Robert W. and Cardoso, M. Cristina and Kolmar, Harald |
| Title: | Cube-octameric silsesquioxane-mediated cargo peptide delivery into living cancer cells. |
| Language: | English |
| Abstract: | Cube octameric silsesquioxanes (COSS) are among the smallest nanoparticles known to date with a diameter of only 0.7 nm. We describe a COSS-based delivery system which allows for the drug targeting in human cells. It comprises a siloxane core with seven pendant aminopropyl groups and a fluorescently labeled peptidic ligand attached to one cage corner via a reversible disulfide bond to ensure its intracellular release. Bimodal amplitude-modulated atomic force microscopy (AFM) experiments revealed the formation of dendritic COSS structures by a self-assembly of single particles on negatively charged surfaces. Nuclear targeting was demonstrated in HeLa cells by selective binding of released p21(Cip1/Waf1)-derived cargo peptide to PCNA, a protein involved in DNA replication and repair. |
| Journal or Publication Title: | Organic & biomolecular chemistry |
| Volume: | 11 |
| Number: | 14 |
| Divisions: | 10 Department of Biology ?? fb10_zoologie ?? 10 Department of Biology > Cell Biology and Epigenetics 11 Department of Materials and Earth Sciences > Material Science > Physics of Surfaces Zentrale Einrichtungen Exzellenzinitiative > Clusters of Excellence > Center of Smart Interfaces (CSI) 11 Department of Materials and Earth Sciences > Material Science Exzellenzinitiative > Clusters of Excellence 11 Department of Materials and Earth Sciences Exzellenzinitiative |
| Date Deposited: | 09 Jan 2013 10:28 |
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