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CMOS-Chip Based Printing System for Combinatorial Synthesis

Cheng, YunChien (2012):
CMOS-Chip Based Printing System for Combinatorial Synthesis.
YunChien Cheng, TU Darmstadt, [Online-Edition: urn:nbn:de:tuda-tuprints-30861],
[Ph.D. Thesis]

Abstract

Peptide arrays, produced through combinatorial synthesis from amino-acid monomers, are an important tool for proteomic research. The arrays enable high throughput screening by exposing a large number of different peptide spots to a solution of target molecules. In particle based synthesis, the monomers are encapsulated into solid particles and deposited on the synthesis spots. The resulting spot density can be much higher than that of liquid based on-spot peptide synthesis. This dissertation presents the construction of a CMOS-chip based “printing system” that automatically prints monomer particles onto a glass slide for combinatorial synthesis of high-density arrays. The monomer particles (~4 μm) are addressed onto the electrode pixels of a CMOS chip, and then printed onto a glass slide by using an electrical field. Afterwards, the particles are melted to release monomers for peptide synthesis. The printing system should print particles onto the slide with high reproducibility and homogeneity. This is achieved with high precision (μm) motors, a slide holder and 3 electrical contact sensors. For ideal printing result, the parameters including particle size, printing voltage, and printing distance are discussed. With printed particles, a peptide synthesis is performed, resulting in an array with 10,000 spots per cm2, which is 100 times more than with conventional method. Additionally, the advantage of the printing system is that the synthesis is not performed on the CMOS chip but on the regular glass slide, which is more chemically robust and suitable for biological applications.

Item Type: Ph.D. Thesis
Erschienen: 2012
Creators: Cheng, YunChien
Title: CMOS-Chip Based Printing System for Combinatorial Synthesis
Language: English
Abstract:

Peptide arrays, produced through combinatorial synthesis from amino-acid monomers, are an important tool for proteomic research. The arrays enable high throughput screening by exposing a large number of different peptide spots to a solution of target molecules. In particle based synthesis, the monomers are encapsulated into solid particles and deposited on the synthesis spots. The resulting spot density can be much higher than that of liquid based on-spot peptide synthesis. This dissertation presents the construction of a CMOS-chip based “printing system” that automatically prints monomer particles onto a glass slide for combinatorial synthesis of high-density arrays. The monomer particles (~4 μm) are addressed onto the electrode pixels of a CMOS chip, and then printed onto a glass slide by using an electrical field. Afterwards, the particles are melted to release monomers for peptide synthesis. The printing system should print particles onto the slide with high reproducibility and homogeneity. This is achieved with high precision (μm) motors, a slide holder and 3 electrical contact sensors. For ideal printing result, the parameters including particle size, printing voltage, and printing distance are discussed. With printed particles, a peptide synthesis is performed, resulting in an array with 10,000 spots per cm2, which is 100 times more than with conventional method. Additionally, the advantage of the printing system is that the synthesis is not performed on the CMOS chip but on the regular glass slide, which is more chemically robust and suitable for biological applications.

Publisher: YunChien Cheng
Divisions: 16 Department of Mechanical Engineering > Institute of Printing Science and Technology (IDD)
16 Department of Mechanical Engineering
Date Deposited: 31 Aug 2012 07:30
Official URL: urn:nbn:de:tuda-tuprints-30861
License: Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
Referees: Doersam, Prof. Dr. Edgar and Schmitz, Prof. Dr. Katja
Refereed / Verteidigung / mdl. Prüfung: 4 July 2012
Alternative Abstract:
Alternative abstract Language
Das Drucksystem soll mit guter Wiederholgenauigkeit und Homogenität Partikel auf Objektträger drucken. Dies wird mit hochpräzisen (µm) Antrieben, einer Trägerhalterung und 3 elektrischen Kontaktsensoren erreicht. Das optimale Druckergebnis wird anhand von Parametern wie der Partikelgröße, der angelegten elektrischen Spannung und dem Abstand zwischen Chip und Objektträger diskutiert. Mit den gedruckten Partikeln wird eine kombinatorische Synthese durchgeführt und somit ein Array mit 10.000 Spots pro cm2 hergestellt, das ist 100-mal mehr als mit herkömmlichen flüssigkeitsbasierten Systemen. Zusätzlich hat das Drucksystem den Vorteil, dass die Synthese nicht direkt auf dem CMOS-Chip durchgeführt wird, sondern auf Glasobjektträgern, die chemikalienbeständiger sind und sich besser für biologische Anwendungen eignen.German
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