Nguyen, Quoc Hung ; Ali, Mubarak ; Neumann, Reinhard ; Ensinger, Wolfgang (2012)
Saccharide/glycoprotein recognition inside synthetic ion channels modified with boronic acid.
In: Sensors and Actuators B: Chemical, 162 (1)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Here, we demonstrate a novel sensing platform for the molecular recognition of monosaccharides (glucose and fructose) and glycoprotein (ovalbumin) using synthetic ion channels fabricated in polymeric membrane. To achieve this, receptor (3-aminophenylboronic acid) is covalently attached with carboxylic groups on the surface and inner walls of the channel. The detection principle rests on the reversible binding occurring between boronic acid and diols of saccharides or glycoprotein molecules, leading to a sensible change in the ionic flux across the nanochannel. In the presence of saccharides or glycoprotein at neutral pH, saccharide-boronic acid complex changes the channel surface from neutral to negative. The binding of ovalbumin onto the channel surface also leads to the reduction of the effective channel diameter, which in turn diminishes the channel conductance. Based on the experimental results, we believe that the presented sensing platform could also be applicable in the area of biomedical sciences for the recognition of cell-surface carbohydrate moieties.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2012 |
| Autor(en): | Nguyen, Quoc Hung ; Ali, Mubarak ; Neumann, Reinhard ; Ensinger, Wolfgang |
| Art des Eintrags: | Bibliographie |
| Titel: | Saccharide/glycoprotein recognition inside synthetic ion channels modified with boronic acid |
| Sprache: | Englisch |
| Publikationsjahr: | 20 Februar 2012 |
| Verlag: | Elsevier Science Publishing Company |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Sensors and Actuators B: Chemical |
| Jahrgang/Volume einer Zeitschrift: | 162 |
| (Heft-)Nummer: | 1 |
| URL / URN: | http://www.sciencedirect.com/science/article/pii/S0925400511... |
| Kurzbeschreibung (Abstract): | Here, we demonstrate a novel sensing platform for the molecular recognition of monosaccharides (glucose and fructose) and glycoprotein (ovalbumin) using synthetic ion channels fabricated in polymeric membrane. To achieve this, receptor (3-aminophenylboronic acid) is covalently attached with carboxylic groups on the surface and inner walls of the channel. The detection principle rests on the reversible binding occurring between boronic acid and diols of saccharides or glycoprotein molecules, leading to a sensible change in the ionic flux across the nanochannel. In the presence of saccharides or glycoprotein at neutral pH, saccharide-boronic acid complex changes the channel surface from neutral to negative. The binding of ovalbumin onto the channel surface also leads to the reduction of the effective channel diameter, which in turn diminishes the channel conductance. Based on the experimental results, we believe that the presented sensing platform could also be applicable in the area of biomedical sciences for the recognition of cell-surface carbohydrate moieties. |
| Freie Schlagworte: | Boronic acid-diols complexation, Ion current rectification, Saccharide sensor, synthetic ion channels, Track-etched nanochannels |
| Fachbereich(e)/-gebiet(e): | 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft > Fachgebiet Materialanalytik 11 Fachbereich Material- und Geowissenschaften > Materialwissenschaft 11 Fachbereich Material- und Geowissenschaften |
| Hinterlegungsdatum: | 06 Mär 2012 14:09 |
| Letzte Änderung: | 05 Mär 2013 09:59 |
| PPN: | |
| Sponsoren: | The authors acknowledge financial support from the Beilstein-Institut, Frankfurt/Main, Germany, within the research collaboration NanoBiC. |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum