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Saccharide/glycoprotein recognition inside synthetic ion channels modified with boronic acid

Nguyen, Quoc Hung and Ali, Mubarak and Neumann, Reinhard and Ensinger, Wolfgang (2012):
Saccharide/glycoprotein recognition inside synthetic ion channels modified with boronic acid.
In: Sensors and Actuators B: Chemical, 162 (1), Elsevier Science Publishing Company, pp. 216-222, [Online-Edition: http://www.sciencedirect.com/science/article/pii/S0925400511...],
[Article]

Abstract

Here, we demonstrate a novel sensing platform for the molecular recognition of monosaccharides (glucose and fructose) and glycoprotein (ovalbumin) using synthetic ion channels fabricated in polymeric membrane. To achieve this, receptor (3-aminophenylboronic acid) is covalently attached with carboxylic groups on the surface and inner walls of the channel. The detection principle rests on the reversible binding occurring between boronic acid and diols of saccharides or glycoprotein molecules, leading to a sensible change in the ionic flux across the nanochannel. In the presence of saccharides or glycoprotein at neutral pH, saccharide-boronic acid complex changes the channel surface from neutral to negative. The binding of ovalbumin onto the channel surface also leads to the reduction of the effective channel diameter, which in turn diminishes the channel conductance. Based on the experimental results, we believe that the presented sensing platform could also be applicable in the area of biomedical sciences for the recognition of cell-surface carbohydrate moieties.

Item Type: Article
Erschienen: 2012
Creators: Nguyen, Quoc Hung and Ali, Mubarak and Neumann, Reinhard and Ensinger, Wolfgang
Title: Saccharide/glycoprotein recognition inside synthetic ion channels modified with boronic acid
Language: English
Abstract:

Here, we demonstrate a novel sensing platform for the molecular recognition of monosaccharides (glucose and fructose) and glycoprotein (ovalbumin) using synthetic ion channels fabricated in polymeric membrane. To achieve this, receptor (3-aminophenylboronic acid) is covalently attached with carboxylic groups on the surface and inner walls of the channel. The detection principle rests on the reversible binding occurring between boronic acid and diols of saccharides or glycoprotein molecules, leading to a sensible change in the ionic flux across the nanochannel. In the presence of saccharides or glycoprotein at neutral pH, saccharide-boronic acid complex changes the channel surface from neutral to negative. The binding of ovalbumin onto the channel surface also leads to the reduction of the effective channel diameter, which in turn diminishes the channel conductance. Based on the experimental results, we believe that the presented sensing platform could also be applicable in the area of biomedical sciences for the recognition of cell-surface carbohydrate moieties.

Journal or Publication Title: Sensors and Actuators B: Chemical
Volume: 162
Number: 1
Publisher: Elsevier Science Publishing Company
Uncontrolled Keywords: Boronic acid-diols complexation, Ion current rectification, Saccharide sensor, synthetic ion channels, Track-etched nanochannels
Divisions: 11 Department of Materials and Earth Sciences > Material Science > Material Analytics
11 Department of Materials and Earth Sciences > Material Science
11 Department of Materials and Earth Sciences
Date Deposited: 06 Mar 2012 14:09
Official URL: http://www.sciencedirect.com/science/article/pii/S0925400511...
Funders: The authors acknowledge financial support from the Beilstein-Institut, Frankfurt/Main, Germany, within the research collaboration NanoBiC.
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