Weigand, Julia E. and Wittmann, Alexander and Suess, Beatrix (2012):
RNA-based networks: using RNA aptamers and ribozymes as synthetic genetic devices.
813, In: Methods in molecular biology (Clifton, N.J.), pp. 157-68, ISSN 1940-6029, [Article]
Abstract
Within the last few years, a set of synthetic riboswitches has been engineered, which expands the toolbox of genetic regulatory devices. Small molecule binding aptamers have been used for the design of such riboswitches by insertion into untranslated regions of mRNAs, exploiting the fact that upon ligand binding the RNA structure interferes either with translation initiation or pre-mRNA splicing in yeast. In combination with self-cleaving ribozymes, aptamers have been used to modulate RNA stability. In this chapter, we discuss the applicability of different aptamers, ways to identify novel genetic devices, the pros and cons of various insertion sites and the application of allosteric ribozymes. Our expertise help to apply synthetic riboswitches to engineer complex genetic circuits.
| Item Type: | Article |
|---|---|
| Erschienen: | 2012 |
| Creators: | Weigand, Julia E. and Wittmann, Alexander and Suess, Beatrix |
| Title: | RNA-based networks: using RNA aptamers and ribozymes as synthetic genetic devices. |
| Language: | English |
| Abstract: | Within the last few years, a set of synthetic riboswitches has been engineered, which expands the toolbox of genetic regulatory devices. Small molecule binding aptamers have been used for the design of such riboswitches by insertion into untranslated regions of mRNAs, exploiting the fact that upon ligand binding the RNA structure interferes either with translation initiation or pre-mRNA splicing in yeast. In combination with self-cleaving ribozymes, aptamers have been used to modulate RNA stability. In this chapter, we discuss the applicability of different aptamers, ways to identify novel genetic devices, the pros and cons of various insertion sites and the application of allosteric ribozymes. Our expertise help to apply synthetic riboswitches to engineer complex genetic circuits. |
| Journal or Publication Title: | Methods in molecular biology (Clifton, N.J.) |
| Volume: | 813 |
| Divisions: | 10 Department of Biology > Synthetic Genetic Circuits 10 Department of Biology |
| Date Deposited: | 22 Feb 2012 09:58 |
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