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Characterization of hammerhead ribozyme reactions.

Kalweit, Anne and Przybilski, Rita and Seehafer, Carsten and de la Peña, Marcos and Hammann, Christian (2012):
Characterization of hammerhead ribozyme reactions.
In: Methods in molecular biology (Clifton, N.J.), pp. 5-20, 848, ISSN 1940-6029,
[Article]

Abstract

Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis.

Item Type: Article
Erschienen: 2012
Creators: Kalweit, Anne and Przybilski, Rita and Seehafer, Carsten and de la Peña, Marcos and Hammann, Christian
Title: Characterization of hammerhead ribozyme reactions.
Language: English
Abstract:

Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis.

Journal or Publication Title: Methods in molecular biology (Clifton, N.J.)
Volume: 848
Divisions: 10 Department of Biology > Ribogenetics
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10 Department of Biology
Date Deposited: 14 Feb 2012 09:12
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