Kalweit, Anne ; Przybilski, Rita ; Seehafer, Carsten ; Peña, Marcos de la ; Hammann, Christian (2012)
Characterization of hammerhead ribozyme reactions.
In: Methods in molecular biology (Clifton, N.J.), 848
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2012 |
| Autor(en): | Kalweit, Anne ; Przybilski, Rita ; Seehafer, Carsten ; Peña, Marcos de la ; Hammann, Christian |
| Art des Eintrags: | Bibliographie |
| Titel: | Characterization of hammerhead ribozyme reactions |
| Sprache: | Englisch |
| Publikationsjahr: | 2012 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Methods in molecular biology (Clifton, N.J.) |
| Jahrgang/Volume einer Zeitschrift: | 848 |
| Kurzbeschreibung (Abstract): | Hammerhead ribozymes are small catalytic RNA motifs ubiquitously present in a large variety of genomes. The reactions catalyzed by these motifs are both their self-scission and the reverse ligation reaction. Here, we describe methods for the generation of DNA templates for the subsequent in vitro transcription of hammerhead ribozymes. This is followed by a description of the preparation of suitable RNA molecules for both reaction types, and their kinetic analysis. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie > Regulatorische RNAs und Ribozyme |
| Hinterlegungsdatum: | 14 Feb 2012 09:12 |
| Letzte Änderung: | 20 Aug 2021 12:49 |
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