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X-ray induced DNA double-strand breaks in coronary CT angiography: Comparison of sequential, low-pitch helical and high-pitch helical data acquisition.

Brand, Michael and Sommer, Matthias and Achenbach, Stephan and Anders, Katharina and Lell, Michael and Löbrich, Markus and Uder, Michael and Kuefner, Michael A. :
X-ray induced DNA double-strand breaks in coronary CT angiography: Comparison of sequential, low-pitch helical and high-pitch helical data acquisition.
In: European journal of radiology, 81 (3) E357-E362. ISSN 1872-7727
[Article] , (2012)

Abstract

BACKGROUND: Aim of this study was to compare DNA double-strand breaks (DSBs) in blood lymphocytes of patients undergoing high-pitch helical, low-pitch helical and sequential coronary CT angiography. METHODS AND RESULTS: 66 patients were examined with various scan protocols and modes (low-pitch helical scan: 100-120kV, 320-438mAs/rot, pitch 0.18-0.39, with or without ECG-pulsing, n=35; prospectively ECG-triggered high-pitch helical scan: 100-120kV, 320-456mAs/rotation, pitch 3.2-3.4, n=19; prospectively ECG-triggered sequential scan: 100-120kV, 150-300mAs or 320-370mAs/rotation, n=12) either using a 64-slice or 128-slice dual-source CT or a 128-slice single source CT scanner. Blood samples were obtained before and 30min after CT and DSBs were analyzed in isolated lymphocytes using γ-H2AX immunofluorescence microscopy. A significant increase of DSBs was measurable 30min after CTA (range 0.01-0.71/cell). CT induced DSBs showed a significant correlation with the estimated effective dose (ρ=0.90, p<0.00001). Both prospectively ECG-triggered sequential (0.10DSBs/cell, 176mGycm, p<0.00001) and high-pitch helical scan protocols (0.03DSBs/cell, 109mGycm, p<0.00001) led to a significant reduction of median DLP and DSB levels compared to low-pitch helical scans (0.34DSBs/cell, 828mGycm). A reduction of the tube voltage resulted in significantly lower whereas additional calcium scoring resulted in elevated DLP and DNA damages (p<0.05 each). CONCLUSION: In coronary CTA, data acquisition protocols have a significant influence on the X-ray induced DSB levels. Using γ-H2AX immunofluorescence microscopy different scan modes in different CT generations can be compared concerning their biological impact.

Item Type: Article
Erschienen: 2012
Creators: Brand, Michael and Sommer, Matthias and Achenbach, Stephan and Anders, Katharina and Lell, Michael and Löbrich, Markus and Uder, Michael and Kuefner, Michael A.
Title: X-ray induced DNA double-strand breaks in coronary CT angiography: Comparison of sequential, low-pitch helical and high-pitch helical data acquisition.
Language: English
Abstract:

BACKGROUND: Aim of this study was to compare DNA double-strand breaks (DSBs) in blood lymphocytes of patients undergoing high-pitch helical, low-pitch helical and sequential coronary CT angiography. METHODS AND RESULTS: 66 patients were examined with various scan protocols and modes (low-pitch helical scan: 100-120kV, 320-438mAs/rot, pitch 0.18-0.39, with or without ECG-pulsing, n=35; prospectively ECG-triggered high-pitch helical scan: 100-120kV, 320-456mAs/rotation, pitch 3.2-3.4, n=19; prospectively ECG-triggered sequential scan: 100-120kV, 150-300mAs or 320-370mAs/rotation, n=12) either using a 64-slice or 128-slice dual-source CT or a 128-slice single source CT scanner. Blood samples were obtained before and 30min after CT and DSBs were analyzed in isolated lymphocytes using γ-H2AX immunofluorescence microscopy. A significant increase of DSBs was measurable 30min after CTA (range 0.01-0.71/cell). CT induced DSBs showed a significant correlation with the estimated effective dose (ρ=0.90, p<0.00001). Both prospectively ECG-triggered sequential (0.10DSBs/cell, 176mGycm, p<0.00001) and high-pitch helical scan protocols (0.03DSBs/cell, 109mGycm, p<0.00001) led to a significant reduction of median DLP and DSB levels compared to low-pitch helical scans (0.34DSBs/cell, 828mGycm). A reduction of the tube voltage resulted in significantly lower whereas additional calcium scoring resulted in elevated DLP and DNA damages (p<0.05 each). CONCLUSION: In coronary CTA, data acquisition protocols have a significant influence on the X-ray induced DSB levels. Using γ-H2AX immunofluorescence microscopy different scan modes in different CT generations can be compared concerning their biological impact.

Journal or Publication Title: European journal of radiology
Volume: 81
Number: 3
Divisions: 10 Department of Biology
10 Department of Biology > Radiation Biology and DNA Repair
Date Deposited: 25 Jan 2012 07:29
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