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The aquaporin TcAQP1 of the desert truffle Terfezia claveryi is a membrane pore for water and CO(2) transport.

Navarro-R Denas, Alfonso and Ruiz-Lozano, Juan Manuel and Kaldenhoff, Ralf and Morte, Asunci N. (2012):
The aquaporin TcAQP1 of the desert truffle Terfezia claveryi is a membrane pore for water and CO(2) transport.
In: Molecular plant-microbe interactions : MPMI, pp. 259-266, 25, (2), ISSN 0894-0282,
[Article]

Abstract

Terfezia claveryi is a hypogeous mycorrhizal fungus belonging to the so-called ?desert truffles?, with a good record as an edible fungus and of considerable economic importance. T. claveryi improves the tolerance to water stress of the host plant Helianthemum almeriense, for which, in field conditions, symbiosis with T. claveryi is valuable for its survival. We have characterized cDNAs from T. claveryi and identified a sequence related with the aquaporin gene family. The full-length sequence was obtained by RACE and was named TcAQP1. This aquaporin gene encoded a functional water channel protein, as demonstrated by heterologous expression assays in Saccharomyces cerevisiae. The mycorrhizal fungal aquaporin increased both water and CO(2) conductivity in the heterologous expression system. The expression patterns of TcAQP1 gene in mycelium, under different water potentials, and in mycorrhizal plants are discussed. The high water conductivity of TcAQP1 could be related with the adaptation of this mycorrhizal fungus to semiarid areas. The CO(2) permeability of TcAQP1 could be involved in the regulation of T. claveryi growth during presymbiotic phases, making it a good candidate for being considered a novel molecular signaling channel in mycorrhizal fungi.

Item Type: Article
Erschienen: 2012
Creators: Navarro-R Denas, Alfonso and Ruiz-Lozano, Juan Manuel and Kaldenhoff, Ralf and Morte, Asunci N.
Title: The aquaporin TcAQP1 of the desert truffle Terfezia claveryi is a membrane pore for water and CO(2) transport.
Language: English
Abstract:

Terfezia claveryi is a hypogeous mycorrhizal fungus belonging to the so-called ?desert truffles?, with a good record as an edible fungus and of considerable economic importance. T. claveryi improves the tolerance to water stress of the host plant Helianthemum almeriense, for which, in field conditions, symbiosis with T. claveryi is valuable for its survival. We have characterized cDNAs from T. claveryi and identified a sequence related with the aquaporin gene family. The full-length sequence was obtained by RACE and was named TcAQP1. This aquaporin gene encoded a functional water channel protein, as demonstrated by heterologous expression assays in Saccharomyces cerevisiae. The mycorrhizal fungal aquaporin increased both water and CO(2) conductivity in the heterologous expression system. The expression patterns of TcAQP1 gene in mycelium, under different water potentials, and in mycorrhizal plants are discussed. The high water conductivity of TcAQP1 could be related with the adaptation of this mycorrhizal fungus to semiarid areas. The CO(2) permeability of TcAQP1 could be involved in the regulation of T. claveryi growth during presymbiotic phases, making it a good candidate for being considered a novel molecular signaling channel in mycorrhizal fungi.

Journal or Publication Title: Molecular plant-microbe interactions : MPMI
Volume: 25
Number: 2
Divisions: 10 Department of Biology > Applied Plant Sciences
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10 Department of Biology
Date Deposited: 12 Jan 2012 09:30
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